Valkova B, Ormerod M G, Moncrieff D, Coleman D V
J Clin Pathol. 1984 Sep;37(9):984-9. doi: 10.1136/jcp.37.9.984.
Smears made from cervical scrapes have been stained immunocytochemically for epithelial membrane antigen using a polyclonal antiserum and two monoclonal antibodies. With the polyclonal antiserum malignant cells and those showing dysplasia consistently expressed the antigen. Normal cells were generally negative, with the exception of some metaplastic cells. The monoclonal antibodies, although they stained the abnormal cells less consistently, gave the same pattern of staining. All three antibodies showed considerable heterogeneity in the intensity of stain. This appears to be a general feature of the expression of this type of epitope in epithelial cells. While the results confirm that an immunohistochemical stain might have potential application for improved diagnostic methods, the staining of metaplastic cells with the presently available antibodies limits the usefulness of an antiserum to epithelial membrane antigen.
取自宫颈刮片的涂片已使用多克隆抗血清和两种单克隆抗体进行上皮膜抗原的免疫细胞化学染色。使用多克隆抗血清时，恶性细胞和显示发育异常的细胞始终表达该抗原。正常细胞通常为阴性，但一些化生细胞除外。单克隆抗体虽然对异常细胞的染色不太一致，但染色模式相同。所有三种抗体在染色强度上均表现出相当大的异质性。这似乎是这种表位在上皮细胞中表达的一个普遍特征。虽然结果证实免疫组织化学染色可能在改进诊断方法方面有潜在应用，但目前可用抗体对化生细胞的染色限制了上皮膜抗原抗血清的实用性。
