Yu Huifang, Wang Jiansheng, Zhao Zhenqing, Sheng Xiaoguang, Shen Yusen, Branca Ferdinando, Gu Honghui
Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Department of Agriculture, Food and Environment, University of Catania, Catania, Italy.
Front Plant Sci. 2019 Jan 29;10:45. doi: 10.3389/fpls.2019.00045. eCollection 2019.
A high-quality genetic map is important for mapping of compound traits. In this study, a genetic map was constructed based on the reference genome TO1000 after specific locus amplified fragment (SLAF) sequencing in a double-haploid segregation population of broccoli, and loci controlling hollow stem trait were identified in the genetic map. The genetic map contains 4,787 SLAF markers, with a mean marker distance of 0.22 cM and the mean sequencing depths of 91.14-fold in the maternal line, 88.97-fold in the paternal line and 17.11-fold in each DH progeny. A locus controlling the hollow stem trait, QHS.C09-2, which could explain 14.1% of the phenotypic variation, was steadily detected on the linkage group nine in the indicated data of 3 years' trials and BLUE analysis. The genetic map could lay an important foundation for mapping of compound traits, and mapping of hollow stem trait would be basis to clone the genes related to hollow stems in broccoli.
高质量的遗传图谱对于复合性状的定位很重要。在本研究中，在西兰花双单倍体分离群体中进行特定位点扩增片段（SLAF）测序后，基于参考基因组TO1000构建了遗传图谱，并在该遗传图谱中鉴定了控制空心茎性状的位点。该遗传图谱包含4787个SLAF标记，平均标记间距为0.22 cM，母本系的平均测序深度为91.14倍，父本系为88.97倍，每个双单倍体后代为17.11倍。在3年试验的指示数据和最佳线性无偏估计（BLUE）分析中，在第9连锁群上稳定检测到一个控制空心茎性状的位点QHS.C09-2，其可解释14.1%的表型变异。该遗传图谱可为复合性状的定位奠定重要基础，而空心茎性状的定位将是克隆西兰花中与空心茎相关基因的基础。
