Mei Jiaqin, Wang Jinhua, Li Yuehua, Tian Shuai, Wei Dayong, Shao Chaoguo, Si Jun, Xiong Qing, Li Jiana, Qian Wei
College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400716, China.
Theor Appl Genet. 2017 Sep;130(9):1953-1959. doi: 10.1007/s00122-017-2936-y. Epub 2017 Jun 20.
The genetic locus for leaf trichome was identified via marker-based mapping and SNP microarray assay, and a functional marker was developed to facilitate the breeding for hairiness in Brassica oleracea. Plant trichomes are involved in various functions particularly in protecting plants against some biotic and abiotic damages. In the present study, an F2 segregating population was developed from the cross between a glabrous cultivated B. oleracea (CC, 2n = 18) and a hairy wild relative, B. incana (CC, 2n = 18). A 1:3 segregation pattern between glabrous and hairy plants was detected among 1063 F2 genotypes, and the locus for hairiness was mapped in a 4.3-cM genetic region using 267 SSR markers among 149 F2 genotypes, corresponding to a 17.6-Mb genomic region on chromosome C01. To narrow the genetic region for hairiness, the Brassica 60 K SNP Bead Chip Arrays were applied to genotype 64 glabrous and 30 hairy F2 plants, resulting in a 1.04-Mb single peak region located in the 17.6-Mb interval. A candidate gene, BoTRY, was identified by qRT-PCR which revealed significant higher expression in glabrous F2 genotypes as compared with that in hairy plants. A cleaved amplified polymorphic site marker was successfully developed to distinguish the sequence variations of BoTRY between hairy and glabrous plants. Our study will be helpful for molecular breeding for hairiness in B. oleracea.
通过基于标记的图谱分析和SNP微阵列分析确定了叶毛状体的遗传位点，并开发了一个功能标记以促进甘蓝毛状性状的育种。植物毛状体参与多种功能，特别是在保护植物免受一些生物和非生物损害方面。在本研究中，从无毛的栽培甘蓝（CC，2n = 18）与有毛的野生近缘种灰白甘蓝（CC，2n = 18）的杂交后代中构建了一个F2分离群体。在1063个F2基因型中检测到无毛和有毛植株之间呈现1:3的分离模式，并利用149个F2基因型中的267个SSR标记将毛状性状位点定位在一个4.3厘摩的遗传区域，该区域对应于C01染色体上17.6兆碱基的基因组区域。为了缩小毛状性状的遗传区域，应用甘蓝60K SNP芯片对64株无毛和30株有毛的F2植株进行基因分型，结果在17.6兆碱基区间内得到一个1.04兆碱基的单峰区域。通过qRT-PCR鉴定出一个候选基因BoTRY，该基因在无毛F2基因型中的表达显著高于有毛植株。成功开发了一个酶切扩增多态性位点标记，以区分有毛和无毛植株中BoTRY的序列变异。我们的研究将有助于甘蓝毛状性状的分子育种。
