Murko Christina, Vieceli Felipe Monteleone, Bronner Marianne
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.
Data Brief. 2018 Nov 27;21:2547-2553. doi: 10.1016/j.dib.2018.11.109. eCollection 2018 Dec.
We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed hybridization to visualize expression of selected transcripts.
我们展示了一个由迁移的鸡胚躯干神经嵴细胞生成的转录组数据集，这些细胞注定会形成外周神经系统的组成部分。利用Sox10E1增强子，其特异性标记沿躯干腹侧途径迁移的神经嵴细胞，我们对电穿孔胚胎进行了荧光激活细胞分选（FACS），以获得这些细胞的纯群体用于文库制备和Illumina测序。结果提供了一份在躯干神经嵴中富集的基因列表。为了验证数据，我们进行了杂交以可视化所选转录本的表达。
