Panda Siva S, Girgis Adel S, Prakash Atish, Khanna Leena, Khanna Pankaj, Shalaby ElSayed M, Fawzy Nehmedo G, Jain Subhash C
Department of Chemistry and Physics, Augusta University, Augusta, GA 30912, USA.
Department of Chemistry, University of Delhi, Delhi 110007, India.
Heliyon. 2018 Nov 30;4(11):e00951. doi: 10.1016/j.heliyon.2018.e00951. eCollection 2018 Nov.
(Buch.-Ham. ex D.Don) Vickery is a native species of India. Different parts of the plant are used for the medicinal purpose by the tribal peoples of south-eastern part of India. However, the biological properties of have not been studied well. The extracts obtained from the bark of were evaluated to determine their protective effect on cognitive impairment and oxidative stress in mice induced by D-galactose using the standard protocol. Different dosages of extract (100, 200, and 300 mg/kg, p.o.) were administered to mice, which were previously treated for six weeks with D-galactose (100 mg/kg s.c.). The D-galactose-induced mice showed significantly impaired cognitive behavior, i.e., oxidative defense, compared to the sham group. Six weeks of treatment with extract (100, 200 and 300 mg/kg, p.o.) considerably improved the cognitive behavior and oxidative impairment of mice compared to the control alone (D-galactose). For the phytochemical investigation, the bark of was successively extracted with dichloromethane and methanol. The chemical constituents of were isolated by multiple column chromatography and characterized by different spectral analyses. ()-Coclaurine (), an alkaloid, was isolated along with two other compounds from the extract; three more compounds were also isolated from the extract of the bark of . All the compounds were isolated for the first time from the bark of , and their structures were established by detailed spectral studies. The structure of compound was also investigated and confirmed by X-ray diffraction and DFT studies. This study highlights the protective effect of bark extract against D-galactose-induced biochemically dysfunction in mice. ()-Coclaurine () was isolated as one of the major components of bark from extract; this compound could be further evaluated for the development of new potential drug candidates.
（布赫-哈姆，引自D. 唐）维克里是印度本土物种。印度东南部的部落民族将该植物的不同部位用于药用。然而，其生物学特性尚未得到充分研究。按照标准方案，对从该植物树皮中提取的提取物进行评估，以确定其对D-半乳糖诱导的小鼠认知障碍和氧化应激的保护作用。给小鼠灌胃不同剂量的提取物（100、200和300毫克/千克），这些小鼠此前已皮下注射D-半乳糖（100毫克/千克）六周。与假手术组相比，D-半乳糖诱导的小鼠表现出明显的认知行为受损，即氧化防御能力受损。与仅用D-半乳糖处理的对照组相比，用提取物（100、200和300毫克/千克，灌胃）处理六周可显著改善小鼠的认知行为和氧化损伤。为进行植物化学研究，用二氯甲烷和甲醇依次提取该植物的树皮。通过多次柱色谱法分离该植物的化学成分，并通过不同的光谱分析进行表征。从该植物提取物中分离出一种生物碱（）-荷包牡丹碱（）以及另外两种化合物；还从该植物树皮的提取物中分离出另外三种化合物。所有化合物均首次从该植物树皮中分离得到，其结构通过详细的光谱研究得以确定。化合物的结构也通过X射线衍射和密度泛函理论研究进行了探究和确认。本研究突出了该植物树皮提取物对D-半乳糖诱导的小鼠生化功能障碍的保护作用。（）-荷包牡丹碱（）作为该植物树皮提取物的主要成分之一被分离出来；该化合物可进一步评估以开发新的潜在药物候选物。
