Rojak S, Wiemer D F, Wille A, Loderstädt U, Wassill L, Hinz R, Hagen R M, Frickmann H
Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany.
Department of Infectious Diseases and Tropical Medicine, Bundeswehr Hospital Hamburg, Hamburg, Germany.
Lett Appl Microbiol. 2019 Jun;68(6):509-513. doi: 10.1111/lam.13130. Epub 2019 Apr 25.
In-house loop-mediated isothermal amplification (LAMP) procedures for the detection of paratyphoid fever-associated bacteria on serovar level were evaluated. Therefore, LAMP primers for Salmonella genus, for two LAMP schemes for S. Paratyphi A, for S. Paratyphi B and for S. Paratyphi C were tested with DNA from culture isolates from strain collections and spiked blood cultures against published PCR protocols targeting the same micro-organisms. Sensitivity and specificity for DNA from culture isolates verified by LAMP ranged from 80·0 to 100·0% and 96·1 to 100·0% vs 65 to 100% and 98·7 to 100% for the PCR approaches. For the spiked blood culture materials, sensitivity and specificity for LAMP ranged from 87·5 to 100·0% and 96·7 to 100·0% vs from 60 to 100% and 98·2 to 100% for PCR. In conclusion, LAMP for paratyphoid fever shows comparable performance characteristics as PCR. Due to its easy application, the procedure is well suited for surveillance purposes in resource-limited settings. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of easy-to-apply, point-of-care-testing-like loop-mediated isothermal amplification (LAMP) for the diagnosis of paratyphoid fever is evaluated. This approach can contribute to low-threshold availability of surveillance options for resource limited settings. Easy-to-teach and easy-to-apply LAMP schemes with similar performance characteristics as PCR are provided. The described test evaluation is of particular use for surveillance and public health experts.
对用于血清型水平检测副伤寒热相关细菌的内部环介导等温扩增（LAMP）程序进行了评估。因此，针对沙门氏菌属、甲型副伤寒沙门氏菌的两种LAMP方案、乙型副伤寒沙门氏菌和丙型副伤寒沙门氏菌的LAMP引物，与来自菌株库培养分离株和加标血培养物的DNA进行了测试，并与针对相同微生物的已发表PCR方案进行了比较。LAMP验证的培养分离株DNA的敏感性和特异性范围为80.0%至100.0%和96.1%至100.0%，而PCR方法的敏感性和特异性范围为65%至100%和98.7%至100%。对于加标血培养材料，LAMP的敏感性和特异性范围为87.5%至100.0%和96.7%至100.0%，而PCR的敏感性和特异性范围为60%至100%和98.2%至100%。总之，用于副伤寒热的LAMP显示出与PCR相当的性能特征。由于其易于应用，该程序非常适合在资源有限的环境中进行监测。研究的意义和影响：评估了使用易于应用的、类似即时检测的环介导等温扩增（LAMP）诊断副伤寒热。这种方法有助于为资源有限的环境提供低门槛的监测选择。提供了易于教授和应用的LAMP方案，其性能特征与PCR相似。所描述的测试评估对监测和公共卫生专家特别有用。
