Department of Genetics, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Animal Production Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
J Anim Physiol Anim Nutr (Berl). 2019 May;103(3):747-755. doi: 10.1111/jpn.13073. Epub 2019 Feb 13.
Heavy metals have enormous variety of deleterious effects on many organs in the body. This study demonstrated the toxic influences of lead on the growth, biochemical, cellular and molecular aspects of developing rabbits. Seventy-five rabbits (New Zealand NZW) were divided into five equal groups as follows; control (C) and four treatment groups (T1-4) orally administered lead acetate solution as follow T1: 20, T2: 30, T3: 50 and T4: 70 mg/kg body weight. Lead resulted in a significant decrease in live body weight, daily body weight gain and feed intake in T3 and T4 compared to those in other groups. Blood haematology measurements such as red blood cells, haematocrit (HCT), mean corpuscular volume, platelet, white blood cells and lymphocytes were significantly influenced by the high level of lead. Oral administration of lead significantly reduced total proteins in the serum. It was observed that the high lead level led to significantly (p < 0.05) increased levels of aspartate aminotransferase, alanine aminotransferase enzymes, urea and creatinine. Four random amplified polymorphic DNA primers polymorphism were detected among the treatment groups. Total number of induced bands (loss or appearance) compared with control group were 4, 10, 10 and 14 bands using primers P1, P2, P3 and P4 respectively. Number of micronuclei showed a dose-response increase and the difference was highly significant especially between control compared with T3 and T4 groups. From our results, we can conclude that exposure of rabbits to lead acetate resulted in negative effects on the growth performance and altered the haematological and biochemical parameters, in addition to its adverse impact on cytological and molecular characterization of animals.
重金属对人体许多器官都有巨大的有害影响。本研究表明，铅对发育中兔子的生长、生化、细胞和分子方面具有毒性影响。75 只新西兰白兔（NZW）被分为五组，每组 15 只，分别为对照组（C）和 4 个实验组（T1-4），通过灌胃给予醋酸铅溶液，剂量分别为：T1：20mg/kg 体重，T2：30mg/kg 体重，T3：50mg/kg 体重，T4：70mg/kg 体重。与其他组相比，T3 和 T4 组的活体重、日增重和采食量显著降低。血液血液学测量，如红细胞、血细胞比容（HCT）、平均红细胞体积、血小板、白细胞和淋巴细胞，都受到高水平铅的显著影响。铅的口服给药显著降低了血清中的总蛋白。观察到高铅水平导致天门冬氨酸氨基转移酶、丙氨酸氨基转移酶、尿素和肌酐的水平显著升高（p<0.05）。在治疗组中检测到 4 个随机扩增的多态性 DNA 引物多态性。与对照组相比，使用引物 P1、P2、P3 和 P4 分别检测到 4、10、10 和 14 条诱导带（缺失或出现）。微核数呈剂量-反应增加，差异非常显著，尤其是对照组与 T3 和 T4 组之间。从我们的结果可以得出结论，暴露于醋酸铅的兔子的生长性能产生负面影响，并改变血液学和生化参数，此外，还对动物的细胞学和分子特征产生不利影响。
