Singh P R, Couvreur M, Decraemer W, Bert W
Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.
J Helminthol. 2019 Feb 14;94:e35. doi: 10.1017/S0022149X19000105.
A survey for slug-associated nematodes in five locations of East and West Flanders in Belgium revealed the presence of one new and six known slug-parasitic nematodes, Agfa flexilis (Dujardin, 1845), Alloionema appendiculatum (Schneider, 1859), Angiostoma dentiferum (Mengert, 1953), Angiostoma limacis (Dujardin, 1845), Angiostoma norvegicum (Ross et al., 2017) and Phasmarhabditis hermaphrodita (Schneider, 1859). Angiostoma norvegicum and P. hermaphrodita are recorded for the first time in Belgium. The six known species are documented by light microscopy (LM) microphotographs and informative DNA sequences. Angiostoma gandavensis n. sp. (Angiostomatidae), discovered from arionid slugs, is described based on light microscopy, scanning electron microscopy (SEM) and molecular data. Based on analyses of D2D3 expansion segment of 28S and 18S rDNA sequences, this new species is found to be related to A. limacis, A. norvegicum, A. margaretae (Ross et al., 2011) and A. milacis (Ivanova and Wilson, 2009). The new species can be distinguished from these others based on morphological characters such as the distinctive mucronate structures at the tail tip of both sexes, presence of lateral ala, reflexed female ovaries and the number and arrangement pattern of male genital papillae.
对比利时东佛兰德省和西佛兰德省五个地点的蛞蝓相关线虫进行的一项调查发现了一种新的和六种已知的寄生在蛞蝓身上的线虫，即柔线杆线虫（Agfa flexilis，杜雅尔丹，1845年）、附肢异线线虫（Alloionema appendiculatum，施奈德，1859年）、具齿血管线虫（Angiostoma dentiferum，门格特，1953年）、蛞蝓血管线虫（Angiostoma limacis，杜雅尔丹，1845年）、挪威血管线虫（Angiostoma norvegicum，罗斯等人，2017年）和雌雄同体小杆线虫（Phasmarhabditis hermaphrodita，施奈德，1859年）。挪威血管线虫和雌雄同体小杆线虫在比利时首次被记录。这六种已知物种通过光学显微镜（LM）显微照片和信息丰富的DNA序列进行了记录。从阿勇蛞蝓中发现的甘达文斯血管线虫新种（Angiostoma gandavensis n. sp.，血管线虫科）基于光学显微镜、扫描电子显微镜（SEM）和分子数据进行了描述。基于对28S和18S rDNA序列的D2D3扩展片段的分析，发现这个新物种与蛞蝓血管线虫、挪威血管线虫、玛格丽塔血管线虫（罗斯等人，2011年）和米拉西斯血管线虫（伊万诺娃和威尔逊，2009年）有关。根据形态特征，如两性尾尖独特的短尖结构、侧翼的存在、雌性卵巢的反折以及雄性生殖乳头的数量和排列模式，可将这个新物种与其他物种区分开来。
