Rolish Singh Phougeishangbam, Lokker Bram, Couvreur Marjolein, Bert Wim, Karssen Gerrit
Department of Biology, Nematology Research Unit, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.
National Plant Protection Organization, Geertjesweg 15, 6706EA Wageningen, The Netherlands.
J Nematol. 2022 Sep 24;54(1):20220037. doi: 10.2478/jofnem-2022-0037. eCollection 2022 Feb.
n. sp. was found associated with holly in the Netherlands and was described based on morphology, morphometrics, rRNA and mitochondrial genes, phylogenetic relationships with other species, host information and geographical distribution. This species can be morphologically diagnosed based on its light brown, slightly obese to obese females with tubercles on cuticle, lateral bands widening into an ovoid field around vulva level, stylet length of 70 μm to 100 μm, outstretched to reflexed ovary, rounded sperm-filled spermatheca, vagina opening into a thick-walled rounded space, absence of vulval flaps and vulva at 89% to 95% of body length, very short tail in all life stages, and a characteristic finger-like tail tip in juveniles (J2). The new species is morphologically closest to but differs based on the absence of a scutellum-like differentiation in the lateral field, the ovaries that can be outstretched or reflexed, and the finger-like tail tip in J2. Furthermore, both species were found to be molecularly distant from one another, found in different habitats, and are associated with different hosts. The high morphological similarity between and and our phylogenetic analyses, revealing that the former is embedded within different clades and thus polyphyletic, provide new evidences for the synonymization of with .
在荷兰发现一种新物种与冬青属植物有关，并基于形态学、形态测量学、rRNA和线粒体基因、与其他物种的系统发育关系、寄主信息和地理分布进行了描述。该物种可根据以下特征进行形态学诊断：浅棕色、略肥胖至肥胖的雌性，角质层有瘤；侧带在阴门水平处扩展成卵形区域；口针长度为70μm至100μm；卵巢伸展至反折；充满精子的受精囊呈圆形；阴道开口于一个厚壁圆形空间；无阴门瓣；阴门位于体长的89%至95%处；所有生命阶段的尾部都非常短；幼虫（J2）的尾尖呈特征性的手指状。新物种在形态上最接近[某物种]，但基于侧区无盾状分化、卵巢可伸展或反折以及J2的手指状尾尖而有所不同。此外，发现这两个物种在分子上彼此距离较远，存在于不同的栖息地，且与不同的寄主相关。[某物种]与[另一物种]之间的高度形态相似性以及我们的系统发育分析表明，前者嵌入不同的进化枝中，因此是多系的，为将[某物种]与[另一物种]同义化提供了新证据。
