Children's Hospital Affiliated to Nanjing Medical University, Department of Gastroenterology, Nanjing, China.
Children's Hospital Affiliated to Nanjing Medical University, Department of Gastroenterology, Nanjing, China.
J Pediatr (Rio J). 2020 May-Jun;96(3):350-355. doi: 10.1016/j.jped.2018.11.012. Epub 2019 Feb 11.
To investigate the differential expression of the thymic stromal lymphopoietin isoforms, short and long, and discern their biological implications under eosinophilic gastroenteritis.
The expression of thymic stromal lymphopoietin and its two isoforms in tissues was assessed by quantitative RT-PCR in healthy controls (n=24) and patients with eosinophilic gastroenteritis (n=17).
Thymic stromal lymphopoietin mRNA was significantly reduced in eosinophilic gastroenteritis when compared with healthy controls (p<0.0001). A significantly lower amount of short thymic stromal lymphopoietin mRNA was observed in eosinophilic gastroenteritis when compared with controls (p<0.05), while a significantly higher amount of long thymic stromal lymphopoietin mRNA was observed in eosinophilic gastroenteritis when compared with controls (p<0.05). Peak eosinophilic count is significantly positively correlated with the expression of long thymic stromal lymphopoietin mRNA in the gastrointestinal mucosal of patients with eosinophilic gastroenteritis (r=0.623, p<0.005), while peak eosinophilic count is significantly negatively correlated with the expression of short thymic stromal lymphopoietin mRNA in the gastrointestinal mucosal of patients with eosinophilic gastroenteritis (r=-0.4474, p<0.05).
Abnormal mucosal thymic stromal lymphopoietin expression may contribute to gastrointestinal mucosa damage in eosinophilic gastroenteritis.
研究胸腺基质淋巴细胞生成素同种型（短型和长型）的差异表达，并探讨其在嗜酸性粒细胞性胃肠炎中的生物学意义。
通过定量 RT-PCR 评估健康对照者（n=24）和嗜酸性粒细胞性胃肠炎患者（n=17）组织中胸腺基质淋巴细胞生成素及其两种同种型的表达。
与健康对照者相比，嗜酸性粒细胞性胃肠炎患者的胸腺基质淋巴细胞生成素 mRNA 显著降低（p<0.0001）。与对照组相比，嗜酸性粒细胞性胃肠炎患者的短型胸腺基质淋巴细胞生成素 mRNA 明显减少（p<0.05），而长型胸腺基质淋巴细胞生成素 mRNA 明显增加（p<0.05）。嗜酸性粒细胞计数峰值与嗜酸性粒细胞性胃肠炎患者胃肠黏膜中长型胸腺基质淋巴细胞生成素 mRNA 的表达呈显著正相关（r=0.623，p<0.005），而嗜酸性粒细胞计数峰值与嗜酸性粒细胞性胃肠炎患者胃肠黏膜中短型胸腺基质淋巴细胞生成素 mRNA 的表达呈显著负相关（r=-0.4474，p<0.05）。
异常的黏膜胸腺基质淋巴细胞生成素表达可能导致嗜酸性粒细胞性胃肠炎的胃肠黏膜损伤。
