Oliver J E, Freer J, Andersen R L, Cox K D, Robinson T L, Fuchs M
Department of Plant Pathology and Plant-Microbe Biology, Cornell University, New York State Agricultural Experiment Station, Geneva 14456.
Department of Horticultural Sciences, Cornell University, New York State Agricultural Experiment Station, Geneva 14456.
Plant Dis. 2009 Jun;93(6):599-606. doi: 10.1094/PDIS-93-6-0599.
A survey for Prunus necrotic ringspot virus (PNRSV) in an orchard of Prunus cerasus cv. Montmorency and Prunus avium cv. Hedelfingen in New York by enzyme-linked immunosorbent assay indicated an eightfold higher infection rate in sour cherry (33%, 32 of 96) than in sweet cherry (4%, 6 of 136) trees. The presence of PNRSV was confirmed by reverse transcription-polymerase chain reaction and amplification of the coat protein (CP) gene in total RNA from infected leaf tissue. Latent infection was prevalent in the majority of trees infected (87%, 33 of 38), while a few of them exhibited shock symptoms or had severely reduced growth (13%, 5 of 38). Asymptomatic PNRSV-infected trees clustered in spatial proximity to symptomatic trees. Sequence analysis of the CP gene (675 bp) indicated a population structure consisting of one predominant molecular variant for 10 isolates and six minor molecular variants for seven isolates. A high sequence identity was found between the CP gene of PNRSV isolates from cherry trees and other isolates from diverse hosts and various geographic origins at the nucleotide and amino acid levels (88 to 100%). Phylogenetic analyses showed a clustering of PNRSV isolates from cherry trees in New York in the predominant group PV-96.
通过酶联免疫吸附测定法对纽约州一个种植酸樱桃品种“蒙特莫伦西”和甜樱桃品种“赫德尔芬根”的果园进行李坏死环斑病毒（PNRSV）调查，结果表明酸樱桃树的感染率（33%，96株中有32株）比甜樱桃树（4%，136株中有6株）高八倍。通过逆转录 - 聚合酶链反应以及对感染叶片组织总RNA中的外壳蛋白（CP）基因进行扩增，证实了PNRSV的存在。在大多数受感染的树（87%，38株中有33株）中潜伏感染很普遍，而其中少数表现出休克症状或生长严重受抑（13%，38株中有5株）。无症状的PNRSV感染树在空间上与有症状的树聚集在一起。CP基因（675 bp）的序列分析表明，其群体结构由10个分离株的一个主要分子变体和7个分离株的6个次要分子变体组成。在核苷酸和氨基酸水平上，樱桃树PNRSV分离株的CP基因与来自不同宿主和不同地理来源的其他分离株之间具有高度的序列同一性（88%至100%）。系统发育分析显示，纽约樱桃树的PNRSV分离株聚集在主要的PV - 96组中。
