Ping Lei, Jian-Jun Chen, Chu-Shu Liao, Guang-Hua Liu, Ming Zhou
Laboratory of Hematology, Hunan Provincial People's Hospital, The First-Affiliated Hospital of Hunan Normal University, Changsha, Hunan Province, China, 410002.
Department of Hematology, Hunan Provincial People's Hospital, The First-Affiliated Hospital of Hunan Normal University, Changsha, Hunan Province, China, 410002.
Oncol Res. 2019 Feb 14. doi: 10.3727/096504018X15412701483326.
Chronic myeloid leukemia (CML) is a rare clonal myeloproliferative malignancy, which is caused by a reciprocal translocation between chromosomes 9 and 22 t(9;22) (q34;q11). Altered circle RNAs (circRNAs) could contribute to leukemogenesis. In this study, we aimed to investigate the expression profiling of circRNAs in CML. We performed circRNA-sequencing to identify differentially expressed circRNAs in CML cells. Furthermore, we found that circ_100053 was significantly upregulated in peripheral blood mononuclear cells (PBMC) and serum samples from CML compared with healthy controls. CircRNA_100053 expression was associated with clinical stage (p=0.037) and BCR/ABL mutant status (p=0.008). High circ_100053 expression predicted a poor outcome of CML patients and imatinib resistance, suggesting the putative role of circ_100053 as an CML biomarker. Our findings provide insights on circRNAs expression in the development and treatment of CML.
慢性髓系白血病（CML）是一种罕见的克隆性骨髓增殖性恶性肿瘤，由9号和22号染色体之间的相互易位t(9;22)(q34;q11)引起。环状RNA（circRNA）的改变可能有助于白血病的发生。在本研究中，我们旨在调查circRNA在CML中的表达谱。我们进行了circRNA测序以鉴定CML细胞中差异表达的circRNA。此外，我们发现与健康对照相比，circ_100053在CML患者的外周血单个核细胞（PBMC）和血清样本中显著上调。CircRNA_100053的表达与临床分期（p=0.037）和BCR/ABL突变状态（p=0.008）相关。circ_100053高表达预示着CML患者预后不良和对伊马替尼耐药，提示circ_100053作为CML生物标志物的潜在作用。我们的研究结果为circRNA在CML发生发展和治疗中的表达提供了见解。
