The Second Hospital of Tianjin Medical University, Tianjin 300211, China.
Tianjin Institute of Urological Surgery, Tianjin Medical University, Tianjin 300070, China.
J Nanosci Nanotechnol. 2019 Jul 1;19(7):3777-3791. doi: 10.1166/jnn.2019.16512.
To determine the short- and long-term antibacterial properties of a novel biomedical titanium alloy to ensure excellent biocompatibility of the TiN/Ag multilayers loaded with different doses of Ag. First, nanosized TiN/Ag multilayers were accumulated onto titanium alloy (Ti-6Al-4V) substrates via multi-arc ion plating. Then, the multilayers were implanted with different doses of silver ions (1×10 ions/cm², 1×10 ions/cm², 5×10 ions/cm², and 5×10 ions/cm²). Both short- and long-term antibacterial properties against and were assessed via unique methods. Additionally, the response and behaviors of MC3T3-E1 and L929 cells on the different surfaces were evaluated by a variety of methods through comparison to a normal matched substrate (Ti-6Al-4V). and analyses revealed that the multilayers containing different doses of Ag ions effectively prevented bacterial adhesion and eliminated the majority of adhered bacteria in the initial period. In addition, the antibacterial activity of each TiN/Ag group improved with time, with the antibacterial rate () ultimately reaching 99% (antibacterial activity: 1 × 10 ions/cm² > 5 × 10 ions/cm² > 1 × 10 ions/cm² > 5 × 10 ions/cm²). All of the samples loaded with Ag exhibited good compatibility, as well as higher cell proliferation and lower apoptosis than the pure Ti-6Al-4V substrates. Considering both bacteriostasis and biocompatibility, 1 × 10 ions/cm² and 5 × 10 ions/cm² are the recommended doses for orthopedic and dental implants. The results indicate that all of the samples loaded with Ag possess excellent biocompatibility and antibacterial activity against common bacteria that cause implantation infection. The samples loaded with Ag can be implanted into soft and hard growing tissues to greatly improve the survival rate of orthopedic and dental implants.
为了确保 TiN/Ag 多层膜负载不同剂量 Ag 的生物医学钛合金具有优异的生物相容性，确定其短期和长期抗菌性能。首先，通过多弧离子镀在钛合金（Ti-6Al-4V）基底上沉积纳米级 TiN/Ag 多层膜。然后，将多层膜注入不同剂量的银离子（1×10 离子/cm²、1×10 离子/cm²、5×10 离子/cm²和 5×10 离子/cm²）。通过独特的方法评估了对 和 的短期和长期抗菌性能。此外，通过与正常匹配的基底（Ti-6Al-4V）进行比较，通过多种方法评估 MC3T3-E1 和 L929 细胞在不同表面的反应和行为。 和 分析表明，含有不同剂量 Ag 离子的多层膜有效地阻止了细菌的黏附，并在初期消除了大部分黏附的细菌。此外，每个 TiN/Ag 组的抗菌活性随时间的延长而提高，抗菌率（）最终达到 99%（抗菌活性：1×10 离子/cm²>5×10 离子/cm²>1×10 离子/cm²>5×10 离子/cm²）。所有负载 Ag 的样品均表现出良好的相容性，细胞增殖率高于纯 Ti-6Al-4V 基底，细胞凋亡率低于纯 Ti-6Al-4V 基底。综合考虑抑菌性和生物相容性，推荐骨科和牙科植入物的负载 Ag 剂量为 1×10 离子/cm²和 5×10 离子/cm²。结果表明，所有负载 Ag 的样品均对引起植入感染的常见细菌具有优异的生物相容性和抗菌活性。负载 Ag 的样品可植入软硬组织中，极大地提高了骨科和牙科植入物的存活率。
