Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China; The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing 100193, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Pestic Biochem Physiol. 2019 Feb;154:46-59. doi: 10.1016/j.pestbp.2018.12.007. Epub 2018 Dec 21.
When any living organism is frequently exposed to any drugs or toxic substances, they evolve different detoxification mechanism to confront with toxicants during absorption and metabolism. Likewise, the insects have evolved detoxification mechanisms as they are frequently exposed to different toxic secondary plant metabolites and commercial insecticides. ABC transporter superfamily is one of the largest and ubiquitous group of proteins which play an important role in phase III of the detoxification process. However, knowledge about this gene family remains largely unknown. To help fill this gap, we have identified a total of 54 ABC transporters in the Helicoverpa armigera genome which are classified into eight subfamilies (A-H) by phylogenetic analysis. The temporal and spatial expression profiles of these 54 ABC transporters throughout H. armigera development stages and seven tissues and their responses to five different insecticides, were investigated using RNA-seq analysis. Furthermore, the mRNA expression of eight selected genes in different tissues and six genes responses to insecticides were confirmed by the quantitative real-time PCR (RT-qPCR). Moreover, H. armigera become more sensitive to abamectin and indoxacarb when P-gp was inhibited. These results provide a foundation for further studies of ABCs in H. armigera.
当任何生物体经常暴露于任何药物或有毒物质时，它们会进化出不同的解毒机制，以在吸收和代谢过程中应对毒物。同样，昆虫也进化出了解毒机制，因为它们经常接触到不同的有毒次生植物代谢物和商业杀虫剂。ABC 转运蛋白超家族是最大和最普遍的蛋白质之一，它们在解毒过程的第三阶段发挥着重要作用。然而，关于这个基因家族的知识仍然知之甚少。为了帮助填补这一空白，我们在棉铃虫基因组中总共鉴定了 54 种 ABC 转运蛋白，通过系统发育分析将它们分为八个亚家族（A-H）。使用 RNA-seq 分析研究了这 54 种 ABC 转运蛋白在棉铃虫发育阶段和七个组织中的时空表达谱及其对五种不同杀虫剂的反应。此外，通过定量实时 PCR（RT-qPCR）证实了不同组织中八个选定基因和六个基因对杀虫剂的 mRNA 表达。此外，当 P-糖蛋白被抑制时，棉铃虫对阿维菌素和茚虫威的敏感性增加。这些结果为进一步研究 ABC 在棉铃虫中的作用奠定了基础。
