Abdel-Kader Maged S, Al Shahrani Khalid S, Alqarni Mohammed H, Salkini Mohammed A, Khamis Elsadig H, Ghabbour Hazem A, Alqasoumi Saleh I
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, 11942 Al Kharj, Saudi Arabia.
Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria 21215, Egypt.
Saudi Pharm J. 2019 Feb;27(2):220-224. doi: 10.1016/j.jsps.2018.11.001. Epub 2018 Nov 8.
Previously, the antimicrobial activity of was traced to benzyl isothiocyanate. In the present study known inactive compounds were isolated from extracts obtained by different solvents including -amyrin, -sitosterol, stigmasterol glucoside, benzyl cyanide and sulphur. However, some inactive compounds were present only in the ethanol and methanol extracts. This observation indicated that these compounds most likely are artifacts resulted from interaction with the solvents used in extraction. Pure benzyl isothiocyanate was kept with different solvents for 72 h and after TLC study they were heated under reflux for 8 h to explore the possibility of interactions. Only solvents with OH groups reacted with benzyl isothiocyanate and gave products similar to those isolated from the alcohol extracts. In conclusion extraction of with hydroxylated solvents will alter the structure of the active compound benzyl isothiocyanate and leads to loss of antimicrobial activity.
先前，[物质名称未给出]的抗菌活性归因于异硫氰酸苄酯。在本研究中，从用不同溶剂获得的提取物中分离出了已知的无活性化合物，包括α-香树脂醇、β-谷甾醇、豆甾醇葡萄糖苷、苄腈和硫。然而，一些无活性化合物仅存在于乙醇和甲醇提取物中。这一观察结果表明，这些化合物很可能是与提取过程中使用的溶剂相互作用产生的假象。将纯异硫氰酸苄酯与不同溶剂放置72小时，经薄层层析研究后，再回流加热8小时，以探究相互作用的可能性。只有含羟基的溶剂与异硫氰酸苄酯发生反应，并生成与从醇提取物中分离出的产物相似的产物。总之，用羟基化溶剂提取[物质名称未给出]会改变活性化合物异硫氰酸苄酯的结构，并导致抗菌活性丧失。
