Ayoub Nahla, Badr Nadia, Al-Ghamdi Saeed S, Alzahrani Arwa, Alsulaimani Rahaf, Nassar Afnan, Qadi Rawabi, Afifi Ibtesam K, Swilam Noha
Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University (UQU), Makkah, Saudi Arabia.
Department of Dental Biomaterials, Faculty of Dentistry, Umm Al-Qura University (UQU), Makkah, Saudi Arabia.
Evid Based Complement Alternat Med. 2021 Dec 27;2021:6333867. doi: 10.1155/2021/6333867. eCollection 2021.
L. (, Siwak) has been used for many centuries as oral hygiene tools, particularly in Saudi Arabia. This study aimed to assess the effectiveness of petroleum ether extract (SPE) as an intracanal bactericidal for endodontic treatment against . Calcium hydroxide Ca(OH) gold standard intracanal medicament was used for comparison.
The gas chromatography mass spectrometry (GC/MS) analysis was carried out to identify the components of SPE. First, the consistency of SPE was accomplished according to ANSI/ADA specification no 57. Forty-five single-rooted mandibular premolars were infected with that of suspension. Colony-forming units (CFU) were counted before the medicaments' application (CFU-1) and after seven days of their applications (CFU-2). Group I: SPE, Group II: positive control Ca(OH), and Group III: saline solution negative control. The microdilution method was applied to determine minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of SPE.
Thirty-two compounds were identified (89.09%), with main components of benzyl isothiocyanate (BITC) (33.32%) and steroids (34%). CFU before and after using SPE and Ca(OH) recorded a statistically significant reduction in bacterial count (=0.006) and (=0.01), respectively. There was an insignificant difference between CFU after using SPE and Ca(OH) (=0.210). On the contrary, comparing both medicaments with the negative control saline group resulted in significant differences, (=0.001) and (=0.007), respectively. Moreover, the equality of minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of SPE is recorded.
This finding could be referred to the high content of bactericidal BITC in synergism with other antimicrobial components, representing 70.71% of SPE. Thus, SPE is a good candidate as an intracanal medicament, which warrants further investigation.
（，西瓦克）几个世纪以来一直被用作口腔卫生工具，尤其是在沙特阿拉伯。本研究旨在评估石油醚提取物（SPE）作为根管内杀菌剂用于牙髓治疗对抗的有效性。使用氢氧化钙Ca(OH)这一根管内药物的金标准进行比较。
进行气相色谱 - 质谱（GC/MS）分析以鉴定SPE的成分。首先，根据ANSI/ADA规范第57号完成SPE的稠度测定。45颗单根下颌前磨牙用悬浮液感染。在药物应用前（CFU-1）和应用七天后（CFU-2）计数菌落形成单位（CFU）。第一组：SPE，第二组：阳性对照Ca(OH)，第三组：生理盐水阴性对照。采用微量稀释法测定SPE的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。
鉴定出32种化合物（89.09%），主要成分是异硫氰酸苄酯（BITC）（33.32%）和类固醇（34%）。使用SPE和Ca(OH)前后CFU记录显示细菌计数分别有统计学显著降低（=0.006）和（=0.01）。使用SPE和Ca(OH)后CFU之间无显著差异（=0.210）。相反，将两种药物与阴性对照生理盐水组比较产生显著差异，分别为（=0.001）和（=0.007）。此外，记录到SPE的最低杀菌浓度（MBC）和最低抑菌浓度（MIC）相等。
这一发现可能归因于杀菌性BITC与其他抗菌成分协同作用的高含量，占SPE的70.71%。因此，SPE是一种很好的根管内药物候选物，值得进一步研究。
