Narayanaswamy Venkatesha, Rah Bilal, Al-Omari Imaddin A, Kamzin Alexander S, Khurshid Hafsa, Muhammad Jibran Sualeh, Obaidat Ihab M, Issa Bashar
Research Institute of Medical & Health Sciences, University of Sharjah, Sharjah P.O. Box 27272, United Arab Emirates.
Department of Physics, Sultan Qaboos University, P.O. Box 36, Muscat 123, Oman.
Pharmaceuticals (Basel). 2024 Mar 1;17(3):327. doi: 10.3390/ph17030327.
The PEG-coated ferrite nanoparticles CoMnZnFeO (X1), CoMnZnFeO (X2), and CoMnZnFeO (X3) were synthesized by the coprecipitation method. The nanoparticles were characterized by XRD, Raman, VSM, XPS, and TEM. The magnetic hyperthermia efficiency (MH) was determined for PEG-coated nanoparticles using an alternating magnetic field (AMF). X2 nanoparticles displayed the highest saturation magnetization and specific absorption rate (SAR) value of 245.2 W/g for 2 mg/mL in a water medium. Based on these properties, X2 nanoparticles were further evaluated for antiproliferative activity against HCT116 cells at an AMF of 495.25 kHz frequency and 350 G strength, using MTT, colony formation, wound healing assays, and flow cytometry analysis for determining the cell viability, clonogenic property, cell migration ability, and cell death of HCT116 cells upon AMF treatment in HCT116 cells, respectively. We observed a significant inhibition of cell viability (2% for untreated control vs. 50% for AMF), colony-forming ability (530 cells/colony for untreated control vs. 220 cells/colony for AMF), abrogation of cell migration (100% wound closure for untreated control vs. 5% wound closure for AMF), and induction of apoptosis-mediated cell death (7.5% for untreated control vs. 24.7% for AMF) of HCT116 cells with respect to untreated control cells after AMF treatment. Collectively, these results demonstrated that the PEG-coated (CoMnZn-FeO) mixed ferrite nanoparticles upon treatment with AMF induced a significant antiproliferative effect on HCT116 cells compared with the untreated cells, indicating the promising antiproliferative potential of the CoMnZnFeO nanoparticles for targeting colorectal cancer cells. Additionally, these results provide appealing evidence that ferrite-based nanoparticles using MH could act as potential anticancer agents and need further evaluation in preclinical models in future studies against colorectal and other cancers.
通过共沉淀法合成了聚乙二醇（PEG）包覆的铁氧体纳米颗粒CoMnZnFeO（X1）、CoMnZnFeO（X2）和CoMnZnFeO（X3）。通过X射线衍射（XRD）、拉曼光谱、振动样品磁强计（VSM）、X射线光电子能谱（XPS）和透射电子显微镜（TEM）对纳米颗粒进行了表征。使用交变磁场（AMF）测定了PEG包覆纳米颗粒的磁热疗效率（MH）。在水介质中，X2纳米颗粒在浓度为2 mg/mL时显示出最高的饱和磁化强度和245.2 W/g的比吸收率（SAR）值。基于这些特性，进一步使用MTT法、集落形成实验、伤口愈合实验以及流式细胞术分析，分别测定在频率为495.25 kHz、强度为350 G的AMF作用下，X2纳米颗粒对HCT116细胞的抗增殖活性，以及HCT116细胞在AMF处理后的细胞活力、克隆形成特性、细胞迁移能力和细胞死亡情况。我们观察到，与未处理的对照相比，AMF处理后HCT116细胞的细胞活力显著受到抑制（未处理对照为2%，AMF处理为50%）、集落形成能力降低（未处理对照为530个细胞/集落，AMF处理为220个细胞/集落）、细胞迁移被阻断（未处理对照伤口完全闭合，AMF处理伤口闭合率为5%）以及凋亡介导的细胞死亡增加（未处理对照为7.5%，AMF处理为24.7%）。总体而言，这些结果表明，与未处理的细胞相比，PEG包覆的（CoMnZn-FeO）混合铁氧体纳米颗粒在AMF处理后对HCT116细胞具有显著的抗增殖作用，表明CoMnZnFeO纳米颗粒在靶向结直肠癌细胞方面具有潜在的抗增殖潜力。此外，这些结果提供了有吸引力的证据，表明基于铁氧体的纳米颗粒利用磁热疗可作为潜在的抗癌剂，在未来针对结直肠癌和其他癌症的临床前模型研究中需要进一步评估。
