Tchinda Alex, Chézeau Laëtitia, Pierson Gaël, Kouitat-Njiwa Richard, Rihn B H, Bravetti Pierre
Jean Lamour Institute, University of Lorraine, UMR 7198, 54011 Nancy, France.
Materials (Basel). 2022 Jul 1;15(13):4655. doi: 10.3390/ma15134655.
The osseointegration of implants is defined as the direct anatomical and functional connection between neoformed living bone and the surface of a supporting implant. The biological compatibility of implants depends on various parameters, such as the nature of the material, chemical composition, surface topography, chemistry and loading, surface treatment, and physical and mechanical properties. In this context, the objective of this study is to evaluate the biocompatibility of rough (Ra = 1 µm) and smooth (Ra = 0 µm) surface conditions of yttria-zirconia (Y-TZP) discs compared to pure zirconia (ZrO) discs by combining a classical toxicological test, morphological observations by SEM, and a transcriptomic analysis on an in vitro model of human Saos-2 bone cells. Similar cell proliferation rates were observed between ZrO and Y-TZP discs and control cells, regardless of the surface topography, at up to 96 h of exposure. Dense cell matting was similarly observed on the surfaces of both materials. Interestingly, only 110 transcripts were differentially expressed across the human transcriptome, consistent with the excellent biocompatibility of Y-TZP reported in the literature. These deregulated transcripts are mainly involved in two pathways, the first being related to "mineral uptake" and the second being the "immune response". These observations suggest that Y-TZP is an interesting candidate for application in implantology.
种植体的骨整合被定义为新形成的活骨与支撑种植体表面之间直接的解剖学和功能连接。种植体的生物相容性取决于各种参数，如材料的性质、化学成分、表面形貌、化学和负荷、表面处理以及物理和机械性能。在此背景下，本研究的目的是通过结合经典毒理学试验、扫描电子显微镜（SEM）形态学观察以及对人Saos-2骨细胞体外模型的转录组分析，评估钇稳定氧化锆（Y-TZP）盘与纯氧化锆（ZrO）盘的粗糙（Ra = 1 µm）和光滑（Ra = 0 µm）表面条件的生物相容性。在长达96小时的暴露时间内，无论表面形貌如何，ZrO盘和Y-TZP盘与对照细胞之间均观察到相似的细胞增殖率。在两种材料的表面均同样观察到致密的细胞铺展。有趣的是，在人类转录组中仅有110个转录本差异表达，这与文献中报道的Y-TZP优异生物相容性一致。这些失调的转录本主要涉及两条途径，第一条与“矿物质摄取”有关，第二条是“免疫反应”。这些观察结果表明，Y-TZP是植入学应用中一个有吸引力的候选材料。
