Department of Trace Element Analysis by Spectroscopy Method, Faculty of Chemistry, Adam Mickiewicz University in Poznan, 89b Umultowska Street, 61-614 Poznan, Poland.
Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska street, 60-479 Poznan, Poland.
Molecules. 2019 Feb 14;24(4):668. doi: 10.3390/molecules24040668.
The main aim of the research was to develop a complementary analytical approach consisting of bespoke speciation analysis and non-targeted speciation analysis of As, Sb, and Cr in flavored bottled drinking water samples using HPLC/ICP-DRC-MS and ESI-MS/MS. The scope of two previously developed analytical procedures, (1) multielemental speciation procedure for As, As, Cr, Sb, and Sb analysis and (2) arsenic speciation procedure for AsB, As, DMA, MMA, and As quantification, was extended to the analysis of a new sample type in terms of bespoke speciation analysis. As for the non-targeted speciation, analysis size exclusion chromatography was used with ICP-MS and a complementary technique, ESI-MS/MS, was used for the organic species of As, Sb, and Cr screening. Full validation of procedures 1 and 2 was conducted. Procedure 1 and 2 were characterized with precision values in the range from 2.5% to 5.5% and from 3.6% to 7.2%, respectively. Obtained recoveries ranged from 97% to 106% and from 99% to 106% for procedures 1 and 2, respectively. Expanded uncertainties calculated for procedures 1 and 2 ranged from 6.1% to 9.4% and from 7.4% to 9.9%, respectively. The applicability of the proposed procedures was tested on bottled drinking water samples. Results for the real samples in procedure 1 were in the range from 0.286 ± 0.027 [μg L] to 0.414 ± 0.039 [μg L] for As, from 0.900 ± 0.083 [μg L] to 3.26 ± 0.30 [μg L] for As, and from 0.201 ± 0.012 [μg L] to 0.524 ± 0.032 [μg L] for Sb. Cr and Sb were not detected in any sample. As for procedure 2, results were in the range from 0.0541 ± 0.0053 [μg L] to 0.554 ± 0.054 [μg L] for AsB. Results for As and As obtained with procedure 2 were in good accordance with results obtained with procedure 1. DMA and MMA were not detected in any sample.
本研究的主要目的是开发一种补充分析方法，该方法由 HPLC/ICP-DRC-MS 和 ESI-MS/MS 用于风味瓶装饮用水样品中砷、锑和铬的定制形态分析和非靶向形态分析组成。此前开发的两种分析程序的范围（1）多元素形态分析程序，用于砷、砷、铬、锑和锑分析，（2）砷形态分析程序，用于砷 B、砷、DMA、MMA 和砷定量，扩展到定制形态分析方面的新型样品类型的分析。对于非靶向形态分析，使用了尺寸排阻色谱法与 ICP-MS，并且互补技术 ESI-MS/MS 用于砷、锑和铬的有机形态筛选。程序 1 和 2 进行了全面验证。程序 1 和 2 的精密度值分别在 2.5%至 5.5%和 3.6%至 7.2%之间。程序 1 和 2 的回收率分别在 97%至 106%和 99%至 106%之间。程序 1 和 2 计算的扩展不确定度分别在 6.1%至 9.4%和 7.4%至 9.9%之间。所提出的程序的适用性已在瓶装饮用水样品上进行了测试。程序 1 中实际样品的结果在 0.286±0.027[μg/L]至 0.414±0.039[μg/L]范围内，用于砷，0.900±0.083[μg/L]至 3.26±0.30[μg/L]用于砷，0.201±0.012[μg/L]至 0.524±0.032[μg/L]用于锑。Cr 和 Sb 未在任何样品中检出。对于程序 2，结果在 0.0541±0.0053[μg/L]至 0.554±0.054[μg/L]范围内，用于砷 B。程序 2 获得的砷和砷的结果与程序 1 获得的结果非常一致。DMA 和 MMA 未在任何样品中检出。
