基于 WS₂量子点固有过氧化物酶样活性的检测 H₂O₂和葡萄糖的化学发光法。

A Chemiluminescent Method for the Detection of H₂O₂ and Glucose Based on Intrinsic Peroxidase-Like Activity of WS₂ Quantum Dots.

机构信息

College of Materials Science and Engineering, Nanjing Forestry University, No. 159, Longpan Road, Nanjing 210037, Jiangsu, China.

Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 51666-16471, Iran.

出版信息

Molecules. 2019 Feb 14;24(4):689. doi: 10.3390/molecules24040689.

Currently, researchers are looking for nanomaterials with peroxidase-like activity to replace natural peroxidase enzymes. For this purpose, WS₂ quantum dots (WS₂ QDs) were synthesized via a solvothermal method, which improved the mimetic behavior. The resulting WS₂ QDs with a size of 1⁻1.5 nm had a high fluorescence emission, dependent on the excitation wavelength. WS₂ QDs with uniform morphology showed a high catalytic effect in destroying H₂O₂. The peroxidase-like activity of synthesized nanostructures was studied in H₂O₂ chemical and electrochemical reduction systems. The mimetic effect of WS₂ QDs was also shown in an H₂O₂⁻rhodamine B (RB) chemiluminescence system. For this aim, a stopped-flow chemiluminescence (CL) detection system was applied. Also, in order to confirm the peroxidase-like effect of quantum dots, colorimetry and electrochemical techniques were used. In the enzymatic reaction of glucose, H₂O₂ is one of the products which can be determined. Under optimum conditions, H₂O₂ can be detected in the concentration range of 0⁻1000 nmol·L, with a detection limit of 2.4 nmol·L. Using this CL assay, a linear relationship was obtained between the intensity of the CL emission and glucose concentration in the range of 0.01⁻30 nmol·L, with a limit of detection (3S) of 4.2 nmol·L.

目前，研究人员正在寻找具有过氧化物酶样活性的纳米材料来替代天然过氧化物酶。为此，通过溶剂热法合成了 WS₂量子点 (WS₂ QDs)，从而改善了模拟行为。所得的 WS₂ QDs 的尺寸为 1⁻1.5nm，具有依赖于激发波长的高荧光发射。具有均匀形态的 WS₂ QDs 在破坏 H₂O₂方面表现出高催化作用。在 H₂O₂化学和电化学还原体系中研究了合成纳米结构的过氧化物酶样活性。WS₂ QDs 的模拟效果也在 H₂O₂-罗丹明 B (RB) 化学发光体系中得到了证明。为此，应用了停流化学发光 (CL) 检测系统。此外，为了确认量子点的过氧化物酶样效应，还使用了比色法和电化学技术。在葡萄糖的酶促反应中，H₂O₂是其中一种产物，可以进行测定。在最佳条件下，可以在 0⁻1000nmol·L 的浓度范围内检测到 H₂O₂，检测限为 2.4nmol·L。使用该 CL 测定法，在 0.01⁻30nmol·L 的范围内，CL 发射强度与葡萄糖浓度之间获得了线性关系，检测限 (3S) 为 4.2nmol·L。