Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan.
Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam.
Molecules. 2019 Feb 15;24(4):691. doi: 10.3390/molecules24040691.
Anti-α-glucosidase (AAG) compounds have received great attention due to their potential use in treating diabetes. In this study, TKU004, an isolated bacterial strain from Taiwanese soil, produced AAG activity in the culture supernatant when squid pens were used as the sole carbon/nitrogen (C/N) source. The protein TKU004P, which was isolated from TKU004, showed stronger AAG activity than acarbose, a commercial anti-diabetic drug (IC = 0.1 mg/mL and 2.02 mg/mL, respectively). The molecular weight of TKU004P, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 29 kDa. High-performance liquid chromatography (HPLC) analysis showed that TKU004P may be a protease that demonstrates AAG activity by degrading yeast α-glucosidase. Among the four chitinous sources of C/N, TKU004P produced the highest AAG activity in the culture supernatant when shrimp head powder was used as the sole source (470.66 U/mL). For comparison, 16 proteases, were investigated for AAG activity but TKU004P produced the highest levels. Overall, the findings suggest that TKU004P could have applications in the biochemical and medicinal fields thanks to its ability to control the activity of α-glucosidase.
由于其在治疗糖尿病方面的潜在用途，抗α-葡萄糖苷酶（AAG）化合物受到了极大的关注。在这项研究中，从台湾土壤中分离出的细菌菌株 TKU004 在以鱿鱼笔作为唯一的碳/氮（C/N）源的培养上清液中产生 AAG 活性。从 TKU004 中分离出的蛋白质 TKU004P 比阿卡波糖（一种商业抗糖尿病药物）具有更强的 AAG 活性（IC = 0.1 mg/mL 和 2.02 mg/mL）。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定的 TKU004P 的分子量为 29 kDa。高效液相色谱（HPLC）分析表明，TKU004P 可能是一种蛋白酶，通过降解酵母α-葡萄糖苷酶来表现出 AAG 活性。在四种 C/N 来源的几丁质中，当虾头粉作为唯一来源时，TKU004P 在培养上清液中产生的 AAG 活性最高（470.66 U/mL）。相比之下，研究了 16 种蛋白酶的 AAG 活性，但 TKU004P 产生的活性最高。总的来说，这些发现表明，由于 TKU004P 能够控制α-葡萄糖苷酶的活性，因此它可能在生化和医学领域有应用前景。
