College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.
Molecules. 2019 Feb 15;24(4):694. doi: 10.3390/molecules24040694.
Clerodane diterpenoids are the main bioactive constituents of and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) process combined with a sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC⁻MS) technique together for the rapid extraction and determination of clerodane diterpenoids in . All calibration curves showed good linearity ( > 0.9943) within the test ranges and the intra- and inter-day precisions and repeatability were all within required limits. This modified HGE⁻UHPLC⁻MS method only took 5 min to extract nine clerodane diterpenoids in and another 12 min to quantify these components. The results indicated that the quantitative analysis based on UHPLC⁻MS was a feasible method for QC of clerodane diterpenoids in , and the findings outlined in the current study also inferred the potential of the method in the QC of clerodane diterpenoids in other complex species of plants.
倍半萜类化合物是 的主要生物活性成分，已被证明具有多种生物活性。然而，关于这些成分的质量控制 (QC) 研究很少。因此，本研究的主要目的是建立一种高效的匀浆提取 (HGE) 工艺，结合灵敏、特异的超高效液相色谱-串联质谱 (UHPLC-MS/MS) 技术，快速提取和测定 中的倍半萜类化合物。所有校准曲线在测试范围内均具有良好的线性关系（>0.9943），日内和日间精密度和重复性均在要求范围内。该改进的 HGE-UHPLC-MS/MS 方法仅需 5 分钟即可从 中提取 9 种倍半萜类化合物，再用 12 分钟即可定量这些成分。结果表明，基于 UHPLC-MS 的定量分析是 中倍半萜类化合物 QC 的一种可行方法，本研究的结果还推断了该方法在其他复杂植物物种中倍半萜类化合物 QC 中的应用潜力。
