Yousefi-Manesh Hasan, Shirooie Samira, Partoazar Alireza, Nikoui Vahid, Estakhri Mohammad Reza Abdollahzadeh, Bakhtiarian Azam
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
J Cell Biochem. 2019 Jul;120(7):11853-11858. doi: 10.1002/jcb.28464. Epub 2019 Feb 15.
It has been proposed carbon tetrachloride (CCl ) intoxication due to the production of free radicals and serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) overload results hepatotoxicity. Phosphatidylserine (PS) has shown antioxidant activity in numerous studies. Therefore, this study was aimed to investigate the effects of PS liposomes treatment against the CCl -induced hepatotoxicity in a rat model. Male Wistar rats were treated with PS (10 mg/kg, oral) or phosphatidylcholine liposomes (PC) (10 mg/kg, oral) for 3 days before CCl (2 ml/kg; ip once on the third day) injection. The serum level of ALT, AST, and ALP were measured. Also, antioxidant assays were performed. Administration of PS with CCl significantly inhibited alterations in the serum levels of AST, ALP ( P < 0.01), and ALT ( P < 0.001) compared with control group. Furthermore, measurement of malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD) levels indicated that PS significantly reduced reactive oxygen species. The results of the present study showed the hepatoprotective effects of PS against CCl -induced hepatotoxicity in rats.
有人提出，四氯化碳（CCl₄）中毒是由于自由基的产生以及血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）水平过载导致肝毒性。在众多研究中，磷脂酰丝氨酸（PS）已显示出抗氧化活性。因此，本研究旨在探讨PS脂质体治疗对大鼠模型中CCl₄诱导的肝毒性的影响。雄性Wistar大鼠在注射CCl₄（2 ml/kg；第三天腹腔注射一次）前3天，用PS（10 mg/kg，口服）或磷脂酰胆碱脂质体（PC）（10 mg/kg，口服）进行治疗。测量血清中ALT、AST和ALP的水平。此外，还进行了抗氧化测定。与对照组相比，PS与CCl₄联合给药显著抑制了AST、ALP（P <0.01）和ALT（P <0.001）血清水平的变化。此外，丙二醛（MDA）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）水平的测量表明，PS显著降低了活性氧。本研究结果表明，PS对大鼠CCl₄诱导的肝毒性具有肝保护作用。
