Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran 13145-784, Iran.
Experimental medicine research center, Tehran University of Medical Sciences, Tehran 13145-784, Iran.
Molecules. 2020 Apr 15;25(8):1816. doi: 10.3390/molecules25081816.
The aim of this research was to examine the effect of the hydroalcoholic extracts from the peel (APE) and pulp (APP) of a traditional apple cultivar from central Italy (Mela Rosa dei Monti Sibillini) on CCl-induced hepatotoxicity in rats. Phytoconstituents were determined by liquid chromatography-mass spectrometry (LC-MS) analysis showing an abundance of proanthocyanidins and flavonol derivatives together with the presence of annurcoic acid in APE. Wistar rats received APE/APP (30 mg/kg oral administration) for three days before CCl injection (2 mL/kg intraperitoneal once on the third day). Treatment with both APE and APP prior to CCl injection significantly decreased the serum levels of aspartate aminotransferase (AST), alkaline phosphatase (ALP) and alanine aminotransferase (ALT) compared to the CCl group. Besides, pretreatment with APE reversed the CCl effects on superoxide dismutase (SOD), myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α) and interleukin-1beta (IL-1β) levels in liver tissue in rats and reduced tissue damage as shown in hematoxylin and eosin staining. These results showed that this ancient Italian apple is worthy of use in nutraceuticals and dietary supplements to prevent and/or protect against liver disorders.
本研究旨在考察来自意大利中部传统苹果品种果皮（APE）和果肉（APP）的水醇提取物对 CCl 诱导的大鼠肝毒性的影响。通过液相色谱-质谱（LC-MS）分析确定植物成分，结果显示 APE 中含有丰富的原花青素和黄酮醇衍生物，以及安努科酸。Wistar 大鼠在 CCl 注射前（第 3 天腹腔内注射 2 mL/kg，一次）连续 3 天给予 APE/APP（30 mg/kg 口服）。与 CCl 组相比，APE 和 APP 预处理显著降低了血清中天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）和丙氨酸转氨酶（ALT）的水平。此外，APE 预处理还逆转了 CCl 对大鼠肝组织中超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平的影响，组织损伤程度如苏木精和伊红染色所示。这些结果表明，这种古老的意大利苹果品种具有作为天然药物和膳食补充剂的使用价值，可用于预防和/或保护肝脏疾病。
