CAS Key Laboratory of Engineering Plastics and CAS Research/Education Center for Excellence in Molecular Sciences, Chinese Academy of Sciences, 100190, Beijing, China.
College of Chemistry, University of Chinese Academy of Sciences, 100049, Beijing, China.
Nat Commun. 2019 Feb 15;10(1):795. doi: 10.1038/s41467-019-08675-3.
Herein, we design and prepare cellulose-based ratiometric fluorescent materials with superior amine-response, which offers the real-time and visual detection of seafood freshness. Through utilizing the reactive hydroxyl groups along cellulose chains, we covalently immobilize the fluorescein isothiocyanate (FITC) as indicator and protoporphyrin IX (PpIX) as internal reference onto cellulose acetate (CA), respectively. Subsequently, a series of dual-emission solid fluorescent materials are achieved by simply blending green emitting CA-FITC with red-emitting CA-PpIX with varying ratios. They exhibit a sensitive, color-responsive, rapid and linear response to ammonia in a wide range of 5.0 ppm to 2.5 × 10 ppm. Benefiting from the excellent solubility and processibility of cellulose derivatives, the as-prepared materials are readily processed into different material forms, including printing ink, coating, flexible film, and nanofibrous membrane. The electrospun nanofibrous membrane is successfully employed as a low-cost, high-contrasting, quick-responsive fluorescent trademark for visual monitoring the freshness of shrimp and crab.
在此，我们设计并制备了基于纤维素的比率型荧光材料，具有优异的胺响应性能，可实现对海鲜新鲜度的实时可视化检测。通过利用纤维素链上的反应性羟基，我们将荧光素异硫氰酸酯 (FITC) 作为指示剂和原卟啉 IX (PpIX) 作为内标物，分别共价固定在醋酸纤维素 (CA) 上。随后，通过简单地将绿色发射的 CA-FITC 与红色发射的 CA-PpIX 以不同比例混合，获得了一系列具有双发射的固态荧光材料。它们对氨具有敏感、颜色响应、快速和线性的响应，响应范围从 5.0 ppm 到 2.5×10 ppm。受益于纤维素衍生物的优异溶解性和加工性能，所制备的材料很容易加工成不同的材料形式，包括印刷油墨、涂层、柔性薄膜和纳米纤维膜。静电纺纳米纤维膜成功地用作低成本、高对比度、快速响应的荧光标记物，用于可视化监测虾和蟹的新鲜度。
