Department of Cell Biology, Bielefeld University, Bielefeld, Germany.
Thin Films & Physics of Nanostructures, Bielefeld University, Bielefeld, Germany.
Nanomedicine. 2019 Apr;17:319-328. doi: 10.1016/j.nano.2019.01.018. Epub 2019 Feb 14.
Bone regeneration is a highly orchestrated process crucial for endogenous healing procedures after accidents, infections or tumor therapy. Changes in surface nanotopography are known to directly affect the formation of osteogenic cell types, although no direct linkage to the endogenous nanotopography of bone was described so far. Here we show the presence of pores of 31.93 ± 0.97 nm diameter on the surface of collagen type I fibers, the organic component of bone, and demonstrate these pores to be sufficient to induce osteogenic differentiation of adult human stem cells. We further applied SiO nanoparticles thermally cross-linked to a nanocomposite to artificially biomimic 31.93 ± 0.97 nm pores, which likewise led to in vitro production of bone mineral by adult human stem cells. Our findings show an endogenous mechanism of directing osteogenic differentiation of adult stem cells by nanotopological cues and provide a direct application using SiO nanocomposites with surface nanotopography biomimicking native bone architecture.
骨再生是一个高度协调的过程，对于事故、感染或肿瘤治疗后的内源性愈合过程至关重要。已知表面纳米形貌的变化会直接影响成骨细胞类型的形成，尽管迄今为止尚未描述与骨的内源性纳米形貌有直接联系。在这里，我们展示了在骨的有机成分 I 型胶原纤维表面存在直径为 31.93±0.97nm 的孔，并且证明这些孔足以诱导成人干细胞的成骨分化。我们进一步应用热交联到纳米复合材料的 SiO 纳米粒子来人工模拟 31.93±0.97nm 的孔，这同样导致了成人干细胞体外产生骨矿物质。我们的研究结果表明，纳米形貌线索可以引导成骨细胞分化，这是一种内源性机制，并提供了一种直接应用，即使用具有模仿天然骨结构的表面纳米形貌的 SiO 纳米复合材料。
