Yang Yue, He Pingya, Li Ning
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei 230032, China.
Evid Based Complement Alternat Med. 2019 Sep 22;2019:1242784. doi: 10.1155/2019/1242784. eCollection 2019.
, a traditional medicinal mushroom, has been historically used in China and other countries of East Asia for the treatment of various diseases. The aim of this study is to investigate the antitumor activity of the extract of (EIB) against hepatocellular carcinoma and the possible mechanism involved. The MTT assay was used to evaluate the proliferative activity of SMMC-7721 cells treated with EIB. Hoechst 33258 and JC-1 staining were used to determine nuclear morphological changes and mitochondrial membrane potential, respectively. Flow cytometry analysis indicated that EIB blocked the cell cycle at the S phase and induced significant apoptosis. EIB increased the protein expression of Bax, cytochrome c, cleaved caspase-3, and decreased Bcl-2 in SMMC-7721. Moreover, EIB induced autophagy, indicated by the increase of autophagy-related protein expression of LC3-II and decrease of p62, and the AMPK/mTOR/ULK1 pathway was involved in the autophagic cell death. , EIB was found to strongly inhibit the growth of tumors in BALB/c nude mice. Our results indicated that might be a potential natural therapeutic agent for liver cancer, as it could induce apoptosis and autophagy in HCC cells.
[某种传统药用蘑菇名称]，一种传统的药用蘑菇，历史上在中国和其他东亚国家被用于治疗各种疾病。本研究的目的是探讨[该蘑菇名称]提取物（EIB）对肝细胞癌的抗肿瘤活性及其可能的作用机制。采用MTT法评估EIB处理的SMMC - 7721细胞的增殖活性。分别用Hoechst 33258和JC - 1染色来确定核形态变化和线粒体膜电位。流式细胞术分析表明EIB使细胞周期阻滞在S期并诱导显著凋亡。EIB增加了SMMC - 7721细胞中Bax、细胞色素c、裂解的caspase - 3的蛋白表达，并降低了Bcl - 2的表达。此外，EIB诱导自噬，表现为自噬相关相关相关蛋白LC3 - II表达增加和p62表达降低，且AMPK/mTOR/ULK1通路参与了自噬性细胞死亡。此外，发现EIB能强烈抑制BALB/c裸鼠体内肿瘤的生长。我们的结果表明，[该蘑菇名称]可能是一种潜在的肝癌天然治疗剂，因为它可以诱导肝癌细胞凋亡和自噬。
