Zhang Tao, Sun Guoqing, Shuai Ming, Ye Jingyu, Huang Jian, Yao Xiaodong, Sun Chengxin, Min Xun
Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, China.
School of Laboratory Medicine, Zunyi Medical University, Zunyi 563006, China.
Food Chem X. 2021 Nov 23;12:100169. doi: 10.1016/j.fochx.2021.100169. eCollection 2021 Dec 30.
Modified citrus pectin (MCP), a commercially available dietary supplement prepared from citrus pectin, contains several different polysaccharide domains, but its primary chemical structure and the binding epitopes that antagonize galectin-3 function remain unclear. In this study, five fractions were isolated from MCP after endo-polygalacturonase degradation (EMCP) and a combination of DEAE-cellulose and Sepharose CL-6B or Sephadex G-75 chromatography. Their primary structures, abilities to inhibit galectin-3-mediated hemagglutination, and antiproliferation activities on MCF-7 and A549 cell lines were studied. Results showed that EMCP-3p, one of the five fractions, was composed of Glc (89.8%), Gal (3.8%), Ara (3.1%), GalA (1.1%), Man (0.9%), and Rha (1.3%) with an average molecular weight of 88.4 KDa, which had the most substantial degree of galectin-3 inhibition with an MIC of 31.25 μg/mL, and it exhibited remarkable cytotoxicity against MCF-7 (36.7%) and A549 (57.4%) cell lines. These results provide new insight into the structure-function relationships of EMCP-derived polysaccharides.
改性柑橘果胶（MCP）是一种由柑橘果胶制备的市售膳食补充剂，含有几种不同的多糖结构域，但其主要化学结构以及拮抗半乳糖凝集素-3功能的结合表位仍不清楚。在本研究中，通过内切多聚半乳糖醛酸酶降解（EMCP）以及DEAE-纤维素和琼脂糖CL-6B或葡聚糖G-75色谱法的组合，从MCP中分离出五个组分。研究了它们的一级结构、抑制半乳糖凝集素-3介导的血细胞凝集的能力以及对MCF-7和A549细胞系的抗增殖活性。结果表明，五个组分之一的EMCP-3p由葡萄糖（89.8%）、半乳糖（3.8%）、阿拉伯糖（3.1%）、半乳糖醛酸（1.1%）、甘露糖（0.9%）和鼠李糖（1.3%）组成，平均分子量为88.4 kDa，其对半乳糖凝集素-3的抑制程度最大，MIC为31.25 μg/mL，并且对MCF-7（36.7%）和A549（57.4%）细胞系表现出显著的细胞毒性。这些结果为EMCP衍生多糖的结构-功能关系提供了新的见解。
