克罗恩病和溃疡性结肠炎患儿非靶向代谢组学和炎症标志物分析——一项初步研究。

Untargeted Metabolomics and Inflammatory Markers Profiling in Children With Crohn's Disease and Ulcerative Colitis-A Preliminary Study.

机构信息

Department of Pediatrics, Gastroenterology, Hepatology, Nutrition and Allergology, Medical University of Bialystok, Bialystok, Poland.

Department of Gastroenterology and Internal Medicine, Medical University of Bialystok, Bialystok, Poland.

出版信息

Inflamm Bowel Dis. 2019 Jun 18;25(7):1120-1128. doi: 10.1093/ibd/izy402.

DOI:10.1093/ibd/izy402

PMID:30772902

Abstract

BACKGROUND

Metabolic profiling might be used to identify disease biomarkers. The aim of our study was to determine the usefulness of untargeted metabolomics analysis to detect differences in serum metabolites between newly diagnosed and untreated pediatric patients with Crohn's disease (CD) or ulcerative colitis (UC) in comparison with a control group (Ctr). Moreover, we investigated the potential of profiling metabolomics and inflammatory markers to improve the noninvasive diagnosis of CD and UC in children.

METHODS

Metabolic fingerprinting of serum samples was estimated with liquid chromatography coupled with mass spectrometry in children with CD (n = 9; median age, 14 years), UC (n = 10; median age, 13.5 years), and controls (n = 10; median age, 12.5 years).

RESULTS

The majority of chemically annotated metabolites belonged to phospholipids and were downregulated in CD and UC compared with the Ctr. Only 1 metabolite, lactosylceramide 18:1/16:0 (LacCer 18:1/16:0), significantly discriminated CD from UC patients. Interestingly, combining LacCer 18:1/16:0 with other inflammatory markers resulted in a significant increase in the area under the curve with the highest specificity and sensitivity.

CONCLUSIONS

Using serum untargeted metabolomics, we have shown that LacCer 18:1/16:0 is a very unique metabolite for CD patients.

摘要

背景

代谢组学分析可能用于鉴定疾病生物标志物。本研究旨在确定非靶向代谢组学分析在鉴定与对照组相比的新诊断且未经治疗的儿童克罗恩病（CD）或溃疡性结肠炎（UC）患者血清代谢物差异方面的作用。此外，我们还研究了代谢组学和炎症标志物分析在改善儿童 CD 和 UC 无创诊断中的潜力。

方法

采用液相色谱-质谱联用技术对 CD 患儿（n=9；中位年龄 14 岁）、UC 患儿（n=10；中位年龄 13.5 岁）和对照组（n=10；中位年龄 12.5 岁）的血清样本进行代谢指纹图谱分析。

结果

大多数被化学注释的代谢物属于磷脂，与对照组相比，在 CD 和 UC 中均下调。只有 1 种代谢物，即半乳糖神经酰胺 18:1/16:0（LacCer 18:1/16:0），可显著区分 CD 与 UC 患者。有趣的是，将 LacCer 18:1/16:0 与其他炎症标志物相结合可显著提高曲线下面积，具有最高的特异性和敏感性。