Department of Clinical Pharmacology, Faculty of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan; Clinical Research Center, Fukuoka Mirai Hospital, Kashiiteriha 3-5-1, Higashi-ku, Fukuoka, 813-0017, Japan.
Department of Social and Behavioral Sciences, Harvard T.H. Chan School of Public Health, 677 Huntington Ave, Boston, MA, 02115, USA; Department of Public Health, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
J Pharmacol Sci. 2019 Mar;139(3):209-214. doi: 10.1016/j.jphs.2019.01.006. Epub 2019 Feb 1.
The aim of this study was to investigate the contribution of gene polymorphisms, in combination with habitual caffeine consumption, to the effect of caffeine intake on hemodynamic and psychoactive parameters. A double-blind, prospective study was conducted with 201 healthy volunteers randomly allocated 2:1 to the caffeinated group (150 mL decaffeinated coffee with additional 200 mg caffeine) or decaffeinated group (150 mL decaffeinated coffee). We measured the changes in blood pressure (BP) and calculation speed upon coffee intake, stratifying with gene polymorphisms, e.g., those in adenosine A receptor (ADORA2A) and cytochrome P450 (CYP) 1A2, and daily caffeine consumption (≤90 mg/day and >90 mg/day). Overall, caffeine intake independently increased BP and calculation speed (p-values < 0.05), irrespective of the polymorphisms. In stratified analysis, a statistical significance within the caffeinated group was observed for the change in systolic BP in the stratum of CYP1A2 polymorphism with daily caffeine consumption ≤90 mg/day: change in systolic BP in the CYP1A2 rs762551 CC group (mean ± SD = 11.8 ± 5.9) was higher than that in the AA/CA group (4.1 ± 5.5). Gene polymorphisms may limitedly modify the effect of caffeine intake on hemodynamic parameters in combination with habitual caffeine consumption.
本研究旨在探讨基因多态性与习惯性咖啡因摄入相结合对咖啡因摄入对血液动力学和精神活性参数影响的贡献。采用双盲、前瞻性研究，将 201 名健康志愿者随机分为咖啡因组（150 毫升脱咖啡因咖啡加 200 毫克咖啡因）或脱咖啡因组（150 毫升脱咖啡因咖啡），2:1 分配。我们测量了咖啡摄入后血压（BP）和计算速度的变化，并对基因多态性进行分层，例如腺苷 A 受体（ADORA2A）和细胞色素 P450（CYP）1A2 的基因多态性，以及每日咖啡因摄入量（≤90mg/天和>90mg/天）。总体而言，无论多态性如何，咖啡因摄入均可独立增加 BP 和计算速度（p 值<0.05）。在分层分析中，在 CYP1A2 多态性与每日咖啡因摄入量≤90mg/天的分层中，观察到收缩压变化的统计学意义：CYP1A2 rs762551 CC 组（平均值±标准差=11.8±5.9）的收缩压变化高于 AA/CA 组（4.1±5.5）。基因多态性可能与习惯性咖啡因摄入相结合，有限地改变咖啡因摄入对血液动力学参数的影响。
