TMEM41B 与 VMP1 共同作用于自噬体的形成。
TMEM41B functions with VMP1 in autophagosome formation.
机构信息
a Department of Biochemistry and Molecular Biology, Graduate School of Medicine , The University of Tokyo , Tokyo , Japan.
出版信息
Autophagy. 2019 May;15(5):922-923. doi: 10.1080/15548627.2019.1582952. Epub 2019 Feb 26.
Abstract
Macroautophagy/autophagy requires many autophagy-related (ATG) proteins. Most of the ATG genes were identified by genetic screening using simple model organisms. Recently, we performed a forward genetic screen in mammalian cells using the CRISPR-Cas9 system and our autophagic flux reporter GFP-LC3-RFP. One of the identified proteins was TMEM41B, an ER-localized multi-spanning membrane protein. TMEM41B has a characteristic transmembrane domain (the VTT domain), which is also found in VMP1, another protein involved in autophagy. Our results show that TMEM41B and VMP1 are physically and functionally associated.
摘要
自噬/自噬需要许多自噬相关（ATG）蛋白。大多数 ATG 基因是通过使用简单的模式生物进行遗传筛选鉴定的。最近，我们使用 CRISPR-Cas9 系统和我们的自噬流报告 GFP-LC3-RFP 在哺乳动物细胞中进行了正向遗传筛选。鉴定出的一种蛋白质是 TMEM41B，一种内质网定位的多跨膜蛋白。TMEM41B 具有特征性的跨膜结构域（VTT 结构域），该结构域也存在于另一种参与自噬的蛋白质 VMP1 中。我们的结果表明 TMEM41B 和 VMP1 具有物理和功能上的关联。
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本文引用的文献
1
Genome-wide CRISPR screen identifies as a gene required for autophagosome formation.全基因组 CRISPR 筛选鉴定为自噬体形成所必需的基因。
J Cell Biol. 2018 Nov 5;217(11):3817-3828. doi: 10.1083/jcb.201804132. Epub 2018 Aug 9.
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