a Department of Biochemistry and Molecular Biology, Graduate School of Medicine , The University of Tokyo , Tokyo , Japan.
Autophagy. 2019 May;15(5):922-923. doi: 10.1080/15548627.2019.1582952. Epub 2019 Feb 26.
Macroautophagy/autophagy requires many autophagy-related (ATG) proteins. Most of the ATG genes were identified by genetic screening using simple model organisms. Recently, we performed a forward genetic screen in mammalian cells using the CRISPR-Cas9 system and our autophagic flux reporter GFP-LC3-RFP. One of the identified proteins was TMEM41B, an ER-localized multi-spanning membrane protein. TMEM41B has a characteristic transmembrane domain (the VTT domain), which is also found in VMP1, another protein involved in autophagy. Our results show that TMEM41B and VMP1 are physically and functionally associated.
自噬/自噬需要许多自噬相关（ATG）蛋白。大多数 ATG 基因是通过使用简单的模式生物进行遗传筛选鉴定的。最近，我们使用 CRISPR-Cas9 系统和我们的自噬流报告 GFP-LC3-RFP 在哺乳动物细胞中进行了正向遗传筛选。鉴定出的一种蛋白质是 TMEM41B，一种内质网定位的多跨膜蛋白。TMEM41B 具有特征性的跨膜结构域（VTT 结构域），该结构域也存在于另一种参与自噬的蛋白质 VMP1 中。我们的结果表明 TMEM41B 和 VMP1 具有物理和功能上的关联。
