Cui Zhifen, Wang Hongyan, Dong Yizhou, Liu Shan-Lu, Wang Qianben
Department of Pathology, Duke University School of Medicine, Durham, NC, United States.
Department of Oncological Sciences, Icahn Genomics Institute, Precision Immunology Institute, Tisch Cancer Institute, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
Front Genome Ed. 2023 Jul 13;5:1231656. doi: 10.3389/fgeed.2023.1231656. eCollection 2023.
Severe respiratory syndrome coronavirus 2 (SARS-CoV-2) and other coronaviruses depend on host factors for the process of viral infection and replication. A better understanding of the dynamic interplay between viral pathogens and host cells, as well as identifying of virus-host dependencies, offers valuable insights into disease mechanisms and informs the development of effective therapeutic strategies against viral infections. This review delves into the key host factors that facilitate or hinder SARS-CoV-2 infection and replication, as identified by CRISPR/Cas9-based screening platforms. Furthermore, we explore CRISPR/Cas13-based gene therapy strategies aimed at targeting these host factors to inhibit viral infection, with the ultimate goal of eradicating SARS-CoV-2 and preventing and treating related coronaviruses for future outbreaks.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）和其他冠状病毒在病毒感染和复制过程中依赖宿主因子。更好地理解病毒病原体与宿主细胞之间的动态相互作用，以及确定病毒-宿主依赖性，有助于深入了解疾病机制，并为开发有效的抗病毒感染治疗策略提供依据。本综述深入探讨了基于CRISPR/Cas9筛选平台确定的促进或阻碍SARS-CoV-2感染和复制的关键宿主因子。此外，我们探索了旨在靶向这些宿主因子以抑制病毒感染的基于CRISPR/Cas13的基因治疗策略，最终目标是根除SARS-CoV-2，并预防和治疗未来爆发的相关冠状病毒。
