Waghamare R N, Paturkar A M, Vaidya V M, Zende R J, Dubal Z N, Dwivedi A, Gaikwad R V
Department of Veterinary Public Health, Bombay Veterinary College, Parel, Mumbai, Maharashtra, India.
Department of Veterinary Public Health, ICAR - Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
Vet World. 2018 Dec;11(12):1682-1688. doi: 10.14202/vetworld.2018.1682-1688. Epub 2018 Dec 16.
The extensive use of antimicrobials in poultry has led to an increase in bacterial multidrug resistance, and the emergence of multidrug-resistant nontyphoidal is a global problem. This study was performed to detect antibiotic-resistant serovars in poultry farming and processing environment.
A total of 956 various samples, comprising 432 farm origin, 324 poultry processing stage wise and environmental, and 154 product processing stages and environmental samples, were collected from poultry farms and processing units located in and around Mumbai city. Of a total of 71 recovered isolates, 42 randomly selected isolates were subjected for antibiotic susceptibility testing by disk diffusion method and serotyping. A total of 31 serotypically confirmed isolates were characterized for the presence of , and CTX-M gene.
Higher resistance was recorded against Doxycycline (100%), followed by Oxytetracycline (97.62%), Neomycin (88.10%), Erythromycin (83.33%), Tetracycline (78.57%), and Ceftizoxime (35.71%). Resistance from 0.00 to 26.19 percent was found to antimicrobials, namely Norfloxacin (26.19%), Ampicillin (21.43%), Azithromycin (21.43%), Ciprofloxacin (19.05%), Colistin (4.76%), Streptomycin (16.67%), Cefotaxime (14.19%), Enrofloxacin (14.29%), Amoxyclav (14.29%), Gentamicin (7.14%), Chloramphenicol (4.76%), Amikacin (4.76%), and Ceftazidime (0.0%). Results demonstrate that the Virchow dominated and all serotypes were found to carry Tetracycline resistance gene , 5 isolates were found to be positive for , whereas none of the isolates were carrying and CTX-M gene.
This study revealed that there is a significant rise of Tetracycline resistance with the presence of gene in spp. which indicates selective pressure for adopting resistance against tetracycline group of antibiotics.
家禽中抗菌药物的广泛使用导致细菌多重耐药性增加，多重耐药非伤寒沙门氏菌的出现是一个全球性问题。本研究旨在检测家禽养殖和加工环境中耐抗生素的沙门氏菌血清型。
从孟买市及其周边的家禽养殖场和加工单位共采集了956份各类样本，包括432份来自农场、324份按家禽加工阶段及环境分类的样本，以及154份产品加工阶段及环境样本。在总共71株分离菌株中，随机选取42株进行纸片扩散法抗生素敏感性试验和血清分型。对总共31株血清型确认的分离菌株进行了blaTEM、blaSHV和CTX-M基因检测。
对多西环素的耐药率最高（100%），其次是土霉素（97.62%）、新霉素（88.10%）、红霉素（83.33%）、四环素（78.57%）和头孢唑肟（35.71%）。对诺氟沙星（26.19%）、氨苄西林（21.43%）、阿奇霉素（21.43%）、环丙沙星（19.05%）、黏菌素（4.76%）、链霉素（16.67%）、头孢噻肟（14.19%）、恩诺沙星（14.29%）、阿莫西林克拉维酸（14.29%）、庆大霉素（7.14%）、氯霉素（4.76%）、阿米卡星（4.76%）和头孢他啶（0.
