Baranwal A, Sonwane A, Chauhan A, Panigrahi M, Sharma A K
These authors contributed equally to this work and should be considered as first authors.
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India.
Iran J Vet Res. 2018 Fall;19(4):283-289.
The present study was conducted to quantify and compare TLR2 (toll-like receptor 2) activity in monocyte-derived macrophages of zebu (Tharparkar) and crossbred (Holstein-Friesian × Jersey × Brown Swiss × Hariana) cattle. The cells were either induced with Pam3CSK4 or kept as control. The TLR2 activity was quantified in terms of IκB-α inhibitory subunit (NFKBIA) messenger RNA (mRNA) copies using real-time, one-step reverse transcription-polymerase chain reaction (RT-PCR). Toll-like receptor 2 activity of induced cells was in the range of 1060421 ± 477937 (n=3) to 3514715 ± 290222 (n=3) copies for Tharparkar cattle (n=7) and in the range of 1365532 ± 47243 (n=3) to 3016510 ± 172340 (n=3) copies for the crossbred cattle (n=7). For uninduced cells, this activity was within the range of 117 ± 51 (n=3) to 293 ± 103 (n=3) copies for the Thraparkar cattle (n=7), and in the range of 182 ± 122 (n=3) to 296 ± 88 (n=3) copies for the crossbred cattle (n=7). The TLR2 activity of induced cells in both groups was found to be significantly higher than that of the respective uninduced cells (P<0.0001). Furthermore, upon comparison, TLR2 activities of induced and uninduced cells of the Tharparkar were not found to be significantly different from those of the crossbred cattle (P=0.8154 and P=0.6670). In the present study, we have quantified and compared, for the first time, TLR2 activity in terms of NFKBIA mRNA copies in monocyte-derived macrophages of Tharparkar and crossbred cattle and found that both have equivalent TLR2 activity.
本研究旨在量化并比较瘤牛（塔帕克牛）和杂交牛（荷斯坦-弗里生×泽西×瑞士褐牛×哈里亚纳牛）单核细胞衍生巨噬细胞中Toll样受体2（TLR2）的活性。细胞分别用Pam3CSK4诱导或作为对照。使用实时一步逆转录聚合酶链反应（RT-PCR），根据IκB-α抑制亚基（NFKBIA）信使核糖核酸（mRNA）拷贝数对TLR2活性进行量化。对于塔帕克牛（n = 7），诱导细胞的Toll样受体2活性在1060421 ± 477937（n = 3）至3514715 ± 290222（n = 3）拷贝之间；对于杂交牛（n = 7），诱导细胞的Toll样受体2活性在1365532 ± 47243（n = 3）至3016510 ± 172340（n = 3）拷贝之间。对于未诱导的细胞，塔帕克牛（n = 7）的该活性在117 ± 51（n = 3）至293 ± 103（n = 3）拷贝之间，杂交牛（n = 7）的该活性在182 ± 122（n = 3）至296 ± 88（n = 3）拷贝之间。发现两组诱导细胞的TLR2活性均显著高于各自未诱导细胞的TLR2活性（P<0.0001）。此外，经比较，塔帕克牛诱导和未诱导细胞的TLR2活性与杂交牛的TLR2活性无显著差异（P = 0.8154和P = 0.6670）。在本研究中，我们首次根据NFKBIA mRNA拷贝数对塔帕克牛和杂交牛单核细胞衍生巨噬细胞中的TLR2活性进行了量化和比较，发现二者具有同等的TLR2活性。
