Shao Xiying, Huang Ping, Shi Lei, Lei Lei, Cao Wenming, Chen Zhanhong, Wang Xiaojia, Zheng Yabing
Clin Lab. 2019 Jan 1;65(1). doi: 10.7754/Clin.Lab.2018.180340.
Recent studies have established non-coding RNAs, which include microRNAs and lncRNAs, are aberrantly expressed in breast cancer. In this study, we explore the expression profile of microRNAs and lncRNAs in ER positive (ER+) breast and paracancerous tissues and define their possible correlations.
We collected ER+ breast cancer patients' and paracancerous tissues from the specimen bank of Zhejiang Cancer Hospital to extract total RNA for obtaining the expression level of microRNAs and lncRNAs by qRT-PCR.
The relative expression results indicated that microRNAs such as MIR-191, MIR-213, MIR-122A had significantly higher expression and MIR-125B-1, MIR-125B-2, MIR-145 had lower expression in ER positive breast cancer compared to normal breast. The interaction of microRNA and lncRNA results exhibited upregulated MIR382-5P and lncRNA 362 in ER+ breast cancer compared to non-cancerous breast. By contrast, MIR222 and NFIA-AS1 are down-regulated. Furthermore, MIR222 and NFIA-AS1 showed different expressions in different TNM stages.
MicroRNAs and lncRNAs are aberrantly expressed in ER+ breast cancer. It is inferred that these microRNAs and lncRNAs may be promising biomarkers for ER positive breast cancer.
最近的研究表明，包括微小RNA（miRNA）和长链非编码RNA（lncRNA）在内的非编码RNA在乳腺癌中异常表达。在本研究中，我们探讨了miRNA和lncRNA在雌激素受体阳性（ER+）乳腺癌组织及癌旁组织中的表达谱，并确定它们之间可能的相关性。
我们从浙江省肿瘤医院标本库收集ER+乳腺癌患者的癌组织及癌旁组织，提取总RNA，通过qRT-PCR获得miRNA和lncRNA的表达水平。
相对表达结果表明，与正常乳腺相比，ER+乳腺癌中MIR-191、MIR-213、MIR-122A等miRNA表达显著升高，而MIR-125B-1、MIR-125B-2、MIR-145表达降低。miRNA与lncRNA的相互作用结果显示，与非癌乳腺相比，ER+乳腺癌中MIR382-5P和lncRNA 362上调。相反，MIR222和NFIA-AS1下调。此外，MIR222和NFIA-AS1在不同TNM分期中表达不同。
miRNA和lncRNA在ER+乳腺癌中异常表达。推测这些miRNA和lncRNA可能是ER阳性乳腺癌有前景的生物标志物。
