Zhang Leilei, Liao Shuijiao, Tan Yuanqing, Wang Gejiao, Wang Dan, Zheng Shixue
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
College of Basic Sciences, Huazhong Agricultural University, Wuhan, 430070, PR China.
Int J Syst Evol Microbiol. 2015 Nov;65(11):4233-4238. doi: 10.1099/ijsem.0.000566. Epub 2015 Aug 26.
A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium, designated strain YLT18T, was isolated from mountain cliff soil of Enshi Grand Canyon in China. The major menaquinone was menaquinone 7 (MK-7) and the predominant fatty acids (>5 %) were iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH) and iso-C15 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, two unknown aminophospholipids, two unknown aminolipids and two unknown polar lipids. The DNA G+C content was 55.4 mol%. According to phylogenetic analysis based on 16S rRNA gene sequences, strain YLT18T was related most closely to Chitinophaga niabensis JS13-10T ( = DSM 24787T) and Chitinophaga cymbidii R156-2T ( = KCTC 23738T), with similarities of 96.7 and 96.2 %, respectively. In addition, strain YLT18T showed obvious differences from the closely related species in terms of esterase (C4) activity, acid production from fructose and rhamnose, and sole carbon source utilization by arabinose and rhamnose. The results from this polyphasic taxonomic study revealed that strain YLT18T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga barathri sp. nov. is proposed. The type strain is YLT18T ( = KCTC 42472T = CCTCC AB 2015054T).
从中国恩施大峡谷的悬崖土壤中分离出一株革兰氏染色阴性、无运动性、杆状、需氧细菌，命名为菌株YLT18T。主要甲基萘醌为甲基萘醌7（MK-7），主要脂肪酸（>5%）为异C15:0、C16:1ω5c、异C17:0 3-OH、总和特征3（C16:1ω7c/异C15:0 2-OH）和异C15:0 3-OH。主要极性脂为磷脂酰乙醇胺、两种未知氨基磷脂、两种未知氨基脂和两种未知极性脂。DNA G+C含量为55.4mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YLT18T与尼亚本斯噬几丁质菌JS13-10T（=DSM 24787T）和大花蕙兰噬几丁质菌R156-2T（=KCTC 23738T）关系最密切，相似性分别为96.7%和96.2%。此外，菌株YLT18T在酯酶（C4）活性、果糖和鼠李糖产酸以及阿拉伯糖和鼠李糖的唯一碳源利用方面与密切相关物种存在明显差异。这项多相分类研究结果表明，菌株YLT18T代表噬几丁质菌属的一个新物种，为此提出新名称巴拉斯噬几丁质菌（Chitinophaga barathri sp. nov.）。模式菌株为YLT18T（=KCTC 42472T = CCTCC AB 2015054T）。
