Kakabadze Zurab, Chakhunashvili David, Gogilashvili Ketevan, Ediberidze Ketevan, Chakhunashvili Konstantine, Kalandarishvili Konstantine, Karalashvili Lia
From the Tbilisi State Medical University, Tbilisi, Georgia.
Exp Clin Transplant. 2019 Jan;17(Suppl 1):92-98. doi: 10.6002/ect.MESOT2018.O29.
In this study, our aim was to create a bioactive wound dressing that combined decellularized and lyophilized human amniotic membrane and freeze-dried rat bone marrow stem cells for the treatment of nonhealing wounds.
For the decellularized human amniotic membrane, sodium dodecyl sulfate and 1% Triton X-100 were used. The mononuclear fraction of bone marrow stem cells was isolated by density gradient centrifugation using Ficoll Paque Plus (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Investigations were conducted on Lewis inbred rats with the radiation wound model (dose of 60 Gy). On day 20 after application of radiation, the skin was excised around the radiation burn. The wound was treated with decellularized human amniotic membrane seeded with and without freeze-dried bone marrow stem cells.
The use of a decellularized amniotic membrane for closing the burn wound increased the rate of healing by 2.5 times; the use of a decellularized amniotic membrane seeded with bone marrow stem cells or freeze-dried bone marrow stem cells increased the rate of wound healing by approximately 4 times.
Administration of freeze-dried bone marrow stem cell may represent a novel therapeutic approach in the treatment of nonhealing wounds and other conditions. We observed no evidence of local or systemic complications related to the procedure. However, further efforts with better protocol design for future studies are needed.
在本研究中，我们的目标是创建一种生物活性伤口敷料，其将脱细胞冻干人羊膜与冻干大鼠骨髓干细胞相结合，用于治疗难愈合伤口。
对于脱细胞人羊膜，使用了十二烷基硫酸钠和1% Triton X - 100。骨髓干细胞的单核部分通过使用Ficoll Paque Plus（美国宾夕法尼亚州匹兹堡市通用电气医疗生物科学公司）进行密度梯度离心分离。在具有辐射伤口模型（剂量为60 Gy）的近交系Lewis大鼠上进行研究。在施加辐射后第20天，切除辐射烧伤周围的皮肤。伤口分别用接种和未接种冻干骨髓干细胞的脱细胞人羊膜进行处理。
使用脱细胞羊膜闭合烧伤伤口使愈合速率提高了2.5倍；使用接种骨髓干细胞或冻干骨髓干细胞的脱细胞羊膜使伤口愈合速率提高了约4倍。
施用冻干骨髓干细胞可能代表了一种治疗难愈合伤口和其他病症的新型治疗方法。我们未观察到与该操作相关的局部或全身并发症的证据。然而，未来研究需要更好的方案设计进行进一步努力。
