Chemical Characterization, Bioactivity and Toxicity of European Flora Plant Extracts in Search for Potential Natural Origin Preservatives.

Consumer demand for natural and healthier products has led to an increasing interest in the bioactive and therapeutic properties of plant extracts. In this study, we evaluated the phenolic compounds profile, bioactivities, and toxicities of plant extracts from eight European flora species, including L., (L.) Hull, L., L., L., L., L., and L. The aim was to identify potential preservatives of natural origin. Phenolic compounds were identified by HPLC-DAD-ESI-MS. Caffeic acid derivatives, ellagitannins, flavonols, and flavones were the major phenolic compounds identified. The total phenolic content varied from 16.0 ± 0.2 () to 123 ± 2 mg/g () of dry extract. All extracts showed antioxidant potential and exhibited activity against some of the microorganisms tested. showed the highest activity in the inhibition of oxidative hemolysis (OxHLIA) assay and , notably, had the lowest IC values in TBARS and DPPH assays, as well as the lowest minimum inhibitory concentration (MIC) values. Regarding in vitro cytotoxicity, in tumor and non-tumor cell lines, although some extracts revealed toxicity against normal cells, it was found that the samples , and might be used against tumor cells since the active concentration is much lower than the one causing toxicity. In vivo acute toxicity tests using suggest low toxicity for most extracts, with LC > 400 mg/L. These results showed the potential of the studied extracts as natural preservatives, given their richness in compounds with bioactive properties, highlight their potential value to the production chain.