Noren Hooten Nicole, Evans Michele K
Laboratory of Epidemiology and Population Science, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
Aging (Albany NY). 2019 Feb 17;11(4):1189-1203. doi: 10.18632/aging.101823.
Emerging evidence indicates that noncoding RNAs play regulatory roles in aging and disease. The functional roles of long noncoding RNAs (lncRNAs) in physiology and disease are not completely understood. Little is known about lncRNAs in the context of human aging and socio-environmental conditions. Microarray profiling of lncRNAs and mRNAs from peripheral blood mononuclear cells from young and old white (n=16) and African American (AA) males (n=16) living above or below poverty from the Healthy Aging in Neighborhoods of Diversity across the Life Span study revealed changes in both lncRNAs and mRNAs with age and poverty status in white males, but not in AA males. We validated lncRNA changes in an expanded cohort (n=40); and changed significantly with age, whereas and changed with poverty. Mitochondrial function and response to DNA damage and stress were pathways enriched in younger individuals. Response to stress, viral infection, and immune signals were pathways enriched in individuals living above poverty. These data show that both human age and a marker of social adversity influence lncRNA expression, which may provide insight about molecular pathways underlying aging and social factors that affect disparities in aging and disease.
新出现的证据表明，非编码RNA在衰老和疾病中发挥调节作用。长链非编码RNA（lncRNA）在生理和疾病中的功能作用尚未完全明确。关于lncRNA在人类衰老和社会环境条件方面的情况知之甚少。来自“跨生命周期多样性社区健康老龄化”研究的年龄在65岁及以上的贫困或非贫困白人（n = 16）男性和非裔美国（AA）男性（n = 16）外周血单核细胞的lncRNA和mRNA微阵列分析显示，lncRNA和mRNA随年龄及贫困状况的变化在白人男性中存在，但在非裔美国男性中未观察到。我们在一个扩大队列（n = 40）中验证了lncRNA的变化； 随年龄显著变化，而 和 随贫困状况变化。线粒体功能以及对DNA损伤和应激的反应是在较年轻个体中富集的通路。对应激、病毒感染和免疫信号的反应是在非贫困个体中富集的通路。这些数据表明，人类年龄和社会逆境标志物均影响lncRNA表达，这可能为衰老的分子通路以及影响衰老和疾病差异的社会因素提供见解。
