Department of Endocrinology, Qiqihar First Hospital, Qiqihar, Heilongjiang, China (mainland).
Department of Endocrinology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
Med Sci Monit. 2019 Feb 19;25:1342-1349. doi: 10.12659/MSM.913463.
BACKGROUND Circular RNAs are important regulators in human cancers, including thyroid carcinoma. The circ_0067934 RNA is reported to participate in hepatocellular carcinoma, esophageal squamous cell carcinoma, and lung cancer. Whether it regulates thyroid carcinoma remains unclear. The purpose of this study was to research potential mechanisms of circ_0067934 in thyroid tumors to provide potential new diagnostic and treatment targets. MATERIAL AND METHODS The expression level of circ_0067934 in thyroid tumors, adjacent tissues, and cell lines was measured by qRT-PCR. The Kaplan-Meier survival curve analysis was used to explore the relationship between circ_0067934 level and survival time of patients. Circ_0067934 was knocked down to research its functional role in thyroid tumors. Cell proliferation was detected by CCK-8 (cell counting kit-8) assay. Migration and invasion were analyzed by Transwell assay. Western blot was applied to analyze the expression of epithelial-mesenchymal-transition (EMT) and PI3K/AKT related proteins. RESULTS Compared with adjacent tissue, circ_0067934 was highly expressed in thyroid tumors. Circ_0067934 expression level was highly expressed in thyroid tumor cell lines. Patients with high expression of circ_0067934 showed lower survival rates. Knockdown of circ_0067934 inhibited cell proliferation, migration, and invasion and also promoted apoptosis. In addition, circ_0067934 knockdown inhibited EMT and PI3K/AKT signaling pathways. CONCLUSIONS circ_0067934 could improve the development of thyroid carcinoma by promoting EMT and PI3K/AKT signaling pathways.
环状 RNA 是人类癌症的重要调控因子，包括甲状腺癌。circ_0067934 RNA 被报道参与肝细胞癌、食管鳞状细胞癌和肺癌。它是否调节甲状腺癌尚不清楚。本研究旨在研究 circ_0067934 在甲状腺肿瘤中的潜在机制，为提供潜在的新的诊断和治疗靶点。
通过 qRT-PCR 测量甲状腺肿瘤、相邻组织和细胞系中 circ_0067934 的表达水平。Kaplan-Meier 生存曲线分析用于探讨 circ_0067934 水平与患者生存时间的关系。敲低 circ_0067934 以研究其在甲状腺肿瘤中的功能作用。CCK-8（细胞计数试剂盒-8）检测细胞增殖。Transwell 分析检测迁移和侵袭。Western blot 用于分析上皮-间充质转化（EMT）和 PI3K/AKT 相关蛋白的表达。
与相邻组织相比，circ_0067934 在甲状腺肿瘤中高表达。circ_0067934 在甲状腺肿瘤细胞系中高表达。circ_0067934 高表达的患者生存率较低。circ_0067934 敲低抑制细胞增殖、迁移和侵袭，并促进凋亡。此外，circ_0067934 敲低抑制 EMT 和 PI3K/AKT 信号通路。
circ_0067934 通过促进 EMT 和 PI3K/AKT 信号通路，可促进甲状腺癌的发展。
