Zou Qinguang, Wang Tiejun, Li Bai, Li Guanghu, Zhang Lixin, Wang Bao, Sun Shaolin
Second Department of Thoracic Surgery, Jilin Provincial Tumor Hospital, Changchun, Jilin 130012, P.R. China.
Department of Orthopedic Traumatology, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
Oncol Lett. 2018 Nov;16(5):5551-5556. doi: 10.3892/ol.2018.9357. Epub 2018 Aug 23.
Previous studies have suggested that circular RNAs (circRNAs) serve crucial functions in various human diseases, including cancer. The results of the present study demonstrated that the expression levels of circ-0067934 were significantly increased in non-small cell lung cancer (NSCLC) tissues and NSCLC cells compared with that in adjacent normal tissues and a normal human bronchial epithelium cell line, as assessed using reverse transcription-quantitative polymerase chain reaction. Increased expression levels of circ-0067934 were associated with lymph node metastasis and advanced tumor stage in patients with NSCLC. Increased levels of circ-0067934 were significantly associated with the poor overall survival (OS) time of patients with NSCLC compared with low circ-0067934 expression levels, as assessed using Kaplan-Meier estimator survival curves and log-rank tests. Additionally, circ-0067934 was identified as an independent risk factor for OS time in patients with NSCLC, as assessed using univariate and multivariate Cox proportional hazards regression models. Additionally, cell proliferation and colony formation assays were employed to assess cell proliferation. The results demonstrated that the proliferative ability was significantly inhibited when circ-0067934 was knocked down in NSCLC cells. Therefore, these results indicated that circ-0067934 may be a predictive marker for the prognosis of NSCLC and a target for the treatment of the disease.
先前的研究表明，环状RNA（circRNA）在包括癌症在内的各种人类疾病中发挥着关键作用。本研究结果表明，与相邻正常组织和正常人支气管上皮细胞系相比，非小细胞肺癌（NSCLC）组织和NSCLC细胞中circ-0067934的表达水平显著升高，这是通过逆转录定量聚合酶链反应评估得出的。circ-0067934表达水平的升高与NSCLC患者的淋巴结转移和肿瘤晚期相关。与低circ-0067934表达水平相比，circ-0067934水平的升高与NSCLC患者较差的总生存期（OS）显著相关，这是通过Kaplan-Meier估计生存曲线和对数秩检验评估得出的。此外，使用单变量和多变量Cox比例风险回归模型评估得出，circ-0067934被确定为NSCLC患者OS时间的独立风险因素。此外，采用细胞增殖和集落形成试验来评估细胞增殖。结果表明，在NSCLC细胞中敲低circ-0067934时，增殖能力受到显著抑制。因此，这些结果表明，circ-0067934可能是NSCLC预后的预测标志物和该疾病治疗的靶点。
