Jiangsu Key Laboratory of Advanced Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.
Department of Phase I Clinical Trial Center, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou 213003, China.
Int J Biol Macromol. 2019 May 15;129:786-791. doi: 10.1016/j.ijbiomac.2019.02.095. Epub 2019 Feb 16.
Sodium alginate (SA) was electrodeposited onto the surface of a glassy carbon electrode (GCE), and then l-lysine (l-Lys), l-alanine (l-Ala) and l-cysteine (l-Cys) were simultaneously introduced to the surface of SA via electrostatic attractions. γ-MnO film was deposited potentiostatically onto the multi-templates immobilized SA from an aqueous mixture of KSO and MnSO. The templates of l-Lys, l-Ala and l-Cys were then oxidized and removed by cyclic voltammetry (CV) while maintaining the crystalline structures of SA and MnO. Finally, the molecularly imprinted SA/MnO was successfully applied in the simultaneous recognition of the isomers of Lys, Ala and Cys by differential pulse voltammetry (DPV). This work opens a new avenue in the construction of SA-based multi-templates molecularly imprinted systems for electrochemical simultaneous recognition of the isomers of several chiral amino acids.
海藻酸钠（SA）被电沉积在玻碳电极（GCE）的表面上，然后通过静电吸引将 l-赖氨酸（l-Lys）、l-丙氨酸（l-Ala）和 l-半胱氨酸（l-Cys）同时引入到 SA 的表面。γ-MnO 薄膜通过恒电位沉积在多模板固定化 SA 上，模板来自 KSO 和 MnSO 的水溶液混合物。然后通过循环伏安法（CV）氧化和去除 l-Lys、l-Ala 和 l-Cys 的模板，同时保持 SA 和 MnO 的结晶结构。最后，分子印迹的 SA/MnO 成功应用于差分脉冲伏安法（DPV）对 Lys、Ala 和 Cys 异构体的同时识别。这项工作为基于 SA 的多模板分子印迹电化学系统的构建开辟了新途径，用于同时识别几种手性氨基酸的异构体。
