Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology , Changzhou University , Changzhou 213164 , China.
Anal Chem. 2019 Oct 1;91(19):12546-12552. doi: 10.1021/acs.analchem.9b03426. Epub 2019 Sep 11.
Chiral recognition of l-amino acids is of significant importance due to the crucial role of l-amino acids in life sciences and pharmaceutics. In this work, a chiral sensor with capability of probing two chiral amino acids by an attractive single-template molecular imprinting strategy is introduced and used in the simultaneous chiral recognition of d/l-alanine (d/l-Ala) and d/l-tyrosine (d/l-Tyr). The assay relies on the hydrolysis of l-alanyl-l-tyrosine dipeptide doped in silica/polypyrrole (SiO/PPy) under acidic conditions, resulting in l-Ala and l-Tyr coimprinted chiral sensor. This work opens up a new avenue for simultaneous chiral sensing of two or more chiral amino acids by incorporating only one template, circumventing the shortcomings encountered with multitemplate molecularly imprinted technology.
手性识别 l-氨基酸具有重要意义，因为 l-氨基酸在生命科学和药物学中起着关键作用。在这项工作中，我们介绍了一种具有通过有吸引力的单模板分子印迹策略探测两种手性氨基酸的能力的手性传感器，并将其用于 d/l-丙氨酸（d/l-Ala）和 d/l-酪氨酸（d/l-Tyr）的同时手性识别。该测定依赖于在酸性条件下掺杂在二氧化硅/聚吡咯（SiO/PPy）中的 l-丙氨酰-l-酪氨酸二肽的水解，导致 l-Ala 和 l-Tyr 共印迹手性传感器。这项工作为通过仅掺入一种模板同时对手性识别两种或更多手性氨基酸开辟了新途径，避免了多模板分子印迹技术所遇到的缺点。
