First Report of Soft Rot Caused by Pectobacterium carotovorum on Pinellia ternata in China.

Pinellia ternata (Thumb.) Breit. (Araceae) is a traditional herb used as an antivomit, anticough, analgesic, and sedative in China for more than 1,000 years. From the summer through fall of 2003 to 2005, a high incidence (approximately 10 to 25%) of disease outbreaks characterized by water-soaked lesions and soft rot with a stinky odor were observed in the cultivated P. ternata in Shanxi Province. Water-soaked lesions were first observed on the stem base and then the plant collapsed, although the upper portion remained asymptomatic. Subsequently, the lesions expanded rapidly over the entire plant. The macerated tuber was usually reduced to a whitish, mushy, and foul-smelling pulp surrounded by undecayed periderm. A Pectobacterium species was consistently recovered from the diseased tubers on nutrient agar media. Koch's postulates were completed by stab inoculating 6-week-old peach- and willow-leaved P. ternata cultivars with the bacterial suspensions (1 × 10 CFU/ml) (1). Ten control plants for each cultivar were inoculated with sterile water. After inoculation, plants were maintained in a growth chamber at 25°C with relative humidity ranging from 80 to 90%. After 2 to 3 days, typical soft rot symptoms were observed on the inoculated plants. A Pectobacterium species was reisolated from the symptomatic tubers while control plants remained healthy. This experiment was repeated in May, July, and September. The pathogenic isolates were identified as typical Pectobacterium carotovorum on the basis of morphological, physiological, and biochemical characteristics (2). The Microlog system (version 3.5; Biolog, Hayward, CA) and Sherlock Microbial Identification System (version 4.5; MIDI Newark, DE) also identified them as Pectobacterium carotovorum on the basis of similarity indices more than 66.9 and 78.2%, respectively. Their identity was then confirmed by sequencing the gene encoding the 16S rRNA (GenBank Accession No DQ785511). To our knowledge, this is the first report of Pectobacterium carotovorum as the cause of soft rot of P. ternata. References: (1) H. R. Azad et al. Plant Dis.84:973, 2000. (2) L. Hauben et al. Syst. Appl. Microbiol. 21:384, 1998.