Duduk B, Bertaccini A
Agriculture Research Institute "Serbia", Pesticide and Environmental Research Centre, Banatska 31b, 11080 Belgrade-Zemun, Serbia.
DiSTA, Patologia Vegetale, Alma Mater Studiorum, University of Bologna, viale Fanin 42, 40127 Bologna, Italy.
Plant Dis. 2006 Oct;90(10):1313-1319. doi: 10.1094/PD-90-1313.
Recurrent epiphytotic outbreaks of a disease of uncertain etiology known as reddening of corn (Zea mays) have occurred in some areas of Serbia during the last 50 years. Affected plants show early and abnormal ripening, dry precociously, and have poor, shriveled grains. Using molecular tools, phytoplasmas were detected in diseased plants and their identity was subsequently deduced as a subgroup 16SrXII-A strain by a variety of supporting assays involving restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-amplified 16S rDNA and tuf gene sequences, selective amplification of phytoplasma DNA using primer pair G35p/m, similarity of 16-23S intergenic spacer region (SR) sequences, and similarity and phylogenetic analysis of 16S rDNA gene sequences. Presence of stolbur phytoplasmas in corn with reddening symptoms is a new finding not only for Serbia: it is the first report of stolbur phytoplasma in this species worldwide.
在过去50年里，塞尔维亚的一些地区反复出现一种病因不明的玉米病害，称为玉米变红病。患病植株表现出早熟和异常成熟，过早干枯，籽粒发育不良且皱缩。利用分子工具，在患病植株中检测到植原体，随后通过多种支持性检测确定其为16SrXII-A亚组菌株，这些检测包括对聚合酶链反应（PCR）扩增的16S rDNA和tuf基因序列进行限制性片段长度多态性（RFLP）分析、使用引物对G35p/m对植原体DNA进行选择性扩增、16 - 23S基因间隔区（SR）序列的相似性分析以及16S rDNA基因序列的相似性和系统发育分析。有变红症状的玉米中存在 stolbur 植原体，这不仅对塞尔维亚来说是一个新发现：这是全世界该物种中首次报道存在stolbur植原体。
