Ćurčić Živko, Kosovac Andrea, Stepanović Jelena, Rekanović Emil, Kube Michael, Duduk Bojan
Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia.
Institute of Pesticides and Environmental Protection, 11080 Belgrade, Serbia.
Microorganisms. 2021 Sep 14;9(9):1950. doi: 10.3390/microorganisms9091950.
Rubbery taproot disease of sugar beet (RTD), associated with ' Phytoplasma solani', appeared in 2020 on an epidemic scale in northern Serbia and southern Slovakia, situated at opposite edges of the Pannonian Plain. In the affected locations where the disease was assessed, symptomatic sugar beets were analysed for phytoplasma infection. Additionally, multilocus sequence analyses of '. P. solani' strains on epidemiologically informative marker genes (, and ) were performed. Symptomatic sugar beets from other countries of the Pannonian Plain (Croatia, Hungary and Austria), one sample from Germany, and red beets from Serbia were included in the analyses. '. P. solani' was detected in sugar beet in all assessed countries, as well as in red beet. Molecular analyses revealed the high genetic variability of '. P. solani' with the presence of all four types (a, b1, b2 and d), 14 genotypes (seven new) and five profiles (one new). The most common multilocus genotype in Serbia, Slovakia, Croatia, and Hungary was dSTOLg (tuf-d/STOL/V2-TA). It was dominant on sites with epidemic RTD outbreaks in the Pannonian Plain and in several sugar beet fields with non-epidemic RTD occurrence suggesting the prevalence of a particular epidemiological pathway during the epidemic's phases.
甜菜橡胶主根病（RTD）与“茄植原体”有关，于2020年在塞尔维亚北部和斯洛伐克南部以流行规模出现，这两个地区位于潘诺尼亚平原的相对边缘。在评估该病的受影响地点，对有症状的甜菜进行了植原体感染分析。此外，还对“茄植原体”菌株在具有流行病学信息的标记基因（、和）上进行了多位点序列分析。分析中纳入了来自潘诺尼亚平原其他国家（克罗地亚、匈牙利和奥地利）的有症状甜菜、一份来自德国的样本以及来自塞尔维亚的红甜菜。在所有评估国家的甜菜以及红甜菜中均检测到了“茄植原体”。分子分析揭示了“茄植原体”的高遗传变异性，存在所有四种类型（a、b1、b2和d）、14种基因型（7种新基因型）和5种图谱（1种新图谱）。在塞尔维亚、斯洛伐克、克罗地亚和匈牙利最常见的多位点基因型是dSTOLg（tuf-d/STOL/V2-TA）。它在潘诺尼亚平原爆发流行RTD的地点以及几个发生非流行RTD的甜菜田占主导地位，这表明在疫情阶段存在特定的流行病学途径。
