Flaitz C M, Hammond H L
Spec Care Dentist. 1988 Mar-Apr;8(2):82-5. doi: 10.1111/j.1754-4505.1988.tb00698.x.
Bone marrow transplant patients (N = 12) with oral lesions consistent with herpes simplex virus (HSV) infection were studied to compare the sensitivity of two rapid cytodiagnostic methods with viral cultures. Routine viral cultures and exfoliative cytology smears were prepared for microscopic evaluation using the Papanicolaou method and an immunoperoxidase staining technique for detection of HSV type 1. Patients with a positive viral culture (N = 10) showed positive staining for HSV, using the immunoperoxidase technique. Multinucleated giant epithelial cells were observed in only seven of the ten positive specimens, using the Papanicolaou method. The results from this preliminary study suggest that the immunoperoxidase method may provide the dentist with a reliable and rapid approach for the diagnosis of intraoral HSV.
对12名患有与单纯疱疹病毒（HSV）感染相符的口腔损害的骨髓移植患者进行了研究，以比较两种快速细胞诊断方法与病毒培养的敏感性。制备了常规病毒培养物和脱落细胞学涂片，使用巴氏染色法和免疫过氧化物酶染色技术进行显微镜评估，以检测1型HSV。病毒培养阳性的患者（n = 10）使用免疫过氧化物酶技术显示HSV染色阳性。使用巴氏染色法，在10个阳性标本中仅7个观察到多核巨上皮细胞。这项初步研究的结果表明，免疫过氧化物酶法可能为牙医提供一种可靠且快速的口腔HSV诊断方法。
