Parker Valerie J, Fascetti Andrea J, Klamer Brett G
Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio.
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California.
J Vet Intern Med. 2019 Mar;33(2):680-685. doi: 10.1111/jvim.15436. Epub 2019 Feb 19.
Proteinuria in dogs with kidney disease can contribute to protein-energy wasting and malnutrition. Little is known about amino acid (AA) status in dogs with protein-losing nephropathy (PLN).
The purpose of our study was to further elucidate AA status in PLN dogs, with the hypothesis that PLN dogs would have altered AA status as compared to healthy dogs.
Thirty client-owned PLN dogs were compared to 10 healthy control dogs.
Prospective observational study. Dogs with PLN that were presented to the teaching hospital were enrolled. Plasma AA profiles were measured using an automated high-performance liquid chromatography AA analyzer.
Compared to control dogs, PLN dogs had significantly lower concentrations of leucine, threonine, histidine, glycine, proline, asparagine, tyrosine, o-hydroxyproline, and serine, as well as sums of both essential and nonessential AA (P < .05). Dogs with PLN had significantly lower ratios of tyrosine-to-phenylalanine and glycine-to-serine (P < .05), and a significantly greater ratio of valine-to-glycine (P < .05).
Dogs with PLN have altered AA status compared to healthy dogs. These findings could have therapeutic implications in determining optimal management of PLN dogs, such as providing AA supplementation along with other standard treatment.
患有肾脏疾病的犬只出现蛋白尿会导致蛋白质-能量消耗和营养不良。关于患有失蛋白性肾病（PLN）的犬只的氨基酸（AA）状态，人们了解甚少。
我们研究的目的是进一步阐明PLN犬只的AA状态，假设与健康犬只相比，PLN犬只的AA状态会发生改变。
将30只客户拥有的PLN犬只与10只健康对照犬只进行比较。
前瞻性观察研究。纳入到教学医院就诊的PLN犬只。使用自动高效液相色谱氨基酸分析仪测量血浆氨基酸谱。
与对照犬只相比，PLN犬只的亮氨酸、苏氨酸、组氨酸、甘氨酸、脯氨酸、天冬酰胺、酪氨酸、邻羟基脯氨酸和丝氨酸的浓度显著降低，必需氨基酸和非必需氨基酸的总和也显著降低（P < 0.05）。PLN犬只的酪氨酸与苯丙氨酸的比值和甘氨酸与丝氨酸的比值显著降低（P < 0.05），缬氨酸与甘氨酸的比值显著升高（P < 0.05）。
与健康犬只相比，PLN犬只的AA状态发生了改变。这些发现可能对确定PLN犬只的最佳管理具有治疗意义，例如在其他标准治疗的同时提供氨基酸补充。
