Department of Neurosurgery, The Affiliated Hospital of Zunyi Medical College, Zunyi, China.
Central Clinical Laboratory, Shenzhen Hospital, Southern Medical University, Shenzhen, China.
Neoplasma. 2019 May 23;66(3):386-396. doi: 10.4149/neo_2018_180606N377.
Long non-coding RNAs (lncRNAs) have been proved to play important roles in carcinogenesis and development of numerous cancers, but their biological functions in glioblastoma remain largely unknown. In this study, we found HOXB-AS1 was highly expressed in human glioblastoma tissues and cell lines, and was associated with survival time of patients. Our results showed HOXB-AS1 knockdown inhibited proliferation via inducing S phase cell cycle arrest, and suppressed migration ability of cells. In terms of mechanism, HOXB-AS1 were mainly located in cytoplasm and functioned as ceRNA via sponging of miR-885-3p. We proved inhibition of miR-885-3p antagonized the effects of HOXB-AS1 konckdown and promoted the proliferation and migration of glioblastoma. Finally, we found the sponging of miR-885-3p by HOXB-AS1 could further affecting the expression of HOXB2. Taken together, we demonstrated that HOXB-AS1/miR-885-3p/HOXB2 axis could regulate the proliferation and migration of glioblastoma, which could serve as a potential biomarker for glioblastoma patients.
长链非编码 RNA（lncRNA）已被证明在多种癌症的发生和发展中发挥重要作用，但它们在神经胶质瘤中的生物学功能仍知之甚少。在这项研究中，我们发现 HOXB-AS1 在人神经胶质瘤组织和细胞系中高表达，并与患者的生存时间相关。我们的结果表明，HOXB-AS1 敲低通过诱导 S 期细胞周期停滞抑制增殖，并抑制细胞迁移能力。就机制而言，HOXB-AS1 主要位于细胞质中，并通过海绵吸附 miR-885-3p 作为 ceRNA 发挥作用。我们证明抑制 miR-885-3p 拮抗了 HOXB-AS1 konckdown 的作用，并促进了神经胶质瘤的增殖和迁移。最后，我们发现 HOXB-AS1 对 miR-885-3p 的海绵吸附作用进一步影响了 HOXB2 的表达。总之，我们证明了 HOXB-AS1/miR-885-3p/HOXB2 轴可以调节神经胶质瘤的增殖和迁移，这可能成为神经胶质瘤患者的潜在生物标志物。
