利用甘薯废渣水解液，在厌氧诱导的nirB 启动子控制下，高效生产工程大肠杆菌菌株中的琥珀酸。
Efficient production of succinic acid in engineered Escherichia coli strains controlled by anaerobically-induced nirB promoter using sweet potato waste hydrolysate.
机构信息
Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China.
出版信息
J Environ Manage. 2019 May 1;237:147-154. doi: 10.1016/j.jenvman.2019.02.041. Epub 2019 Feb 19.
Succinic acid has attracted interest worldwide as a precursor of many industrially crucial chemicals. Biosynthesis of succinic acid from biomass is developing as an environmentally friendly strategy now. Conversion of sweet potato waste (SPW) to succinic acid could implement high-value utilization of biomass, cut cost of the fermentation process and reduce the pollution of environment. Engineered Escherichia coli (E. coli) strain HD134 under the control of anaerobically-induced nirB promoter from Salmonella enterica (P) could produce about 16.30 g/L succinic acid with a yield of 0.83 g/g after 48 h on glucose. With SPW hydrolysate as the substrate, 18.65 g/L succinic acid with a yield of 0.94 g/g after 48 h fermentation achieved. Compared to SD134 under Trc control induced with Isopropyl β-D-Thiogalactoside (IPTG), this concentration and yield represented an 8.56% and 6.82% increase, respectively. The use of anaerobically-induced P not only could attain higher production of succinic acid than IPTG-induced Trc promoter, but omit cost of expensive exogenous inducers. The efficient production of succinic acid from SPW was firstly studied by anaerobically-induced P control, which achieved relative lower cost compared to glucose as substrate and IPTG as the inducer. This novel fermentation process conduces to the cosmically industrial succinic acid bioproduction.
琥珀酸作为许多工业关键化学品的前体，已引起全球关注。现在，从生物质合成琥珀酸作为一种环保策略正在发展。将甘薯废弃物（SPW）转化为琥珀酸，可以实现生物质的高价值利用，降低发酵过程的成本，并减少环境污染。在沙门氏菌属（P）的厌氧诱导nirB 启动子的控制下，工程大肠杆菌（E. coli）菌株 HD134 可以在 48 小时内产生约 16.30 g/L 的琥珀酸，产率为 0.83 g/g，葡萄糖为底物。利用 SPW 水解物作为底物，发酵 48 小时后可获得 18.65 g/L 的琥珀酸，产率为 0.94 g/g。与在 Trc 控制下、异丙基-β-D-硫代半乳糖苷（IPTG）诱导的 SD134 相比，该浓度和产率分别提高了 8.56%和 6.82%。厌氧诱导 P 的使用不仅可以获得比 IPTG 诱导 Trc 启动子更高的琥珀酸产量，而且可以省略昂贵的外源性诱导剂的成本。首次通过厌氧诱导 P 控制研究了从 SPW 高效生产琥珀酸，与以葡萄糖为底物和 IPTG 为诱导剂相比，成本相对较低。这种新型发酵工艺有助于实现宇宙工业规模的琥珀酸生物生产。
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